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because silica is the best adsorbent used in column chromatography it almost supoort polar and non polar substances which areto be seprated....another reason is that it also seprate those substances which has intermediate polarities......
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Why are silica gels used in chromatography?
to separate the organic compounds
What carrier gas is used in packed columns of gas chromatography?
Nitrogen is usually used.
Why do open tubular columns provide greater resolution than packed columns in gas chromatography?
Open tubular columns provide greater resolution than packed columns in gas chromatography because of their large retention time.
What is the difference between vacuum chromatography and flash chromatography?
Flash chromatography uses pressure (under 10 psi) to pump solvent down a column at a rate faster than gravity would provide. Vacuum chromatography uses a vacuum at the bottom of the column to pull solvent through. Both can be performed with standard glass columns, but usually vacuum chromatography is done with a silica filled vacuum funnel instead as a rough purification technique.
When you use c8 and when c18 column?
Both C8 and C18 columns are considered as examples of reversed phase liquid chromatography (RP). The stationary phase here is seen as a thin film of non-polar liquid phase that has been designed to be chemically anchored to an inert material (Silica gel particles). The non-polar layer is chemically linked to the silica particles surface by reaction with the polar silanol groups on the stationary phase surface and so rendering them (less polar or non-polar), The difference between the two columns will be in the length of the carbon-chain attached to the silica surface. Acoordingly C8 hplc columns have packing material composed of silica particles attached to C8 carbon units while C18 will, of course, have packing materials coated with C18 hydrophobic units. Categorically both are reversed phase but C18 columns will definitely be more "hydrophobic" rather than the C8 columns. See also the links below.
Why are silica gels used in chromatography?
to separate the organic compounds
What carrier gas is used in packed columns of gas chromatography?
Nitrogen is usually used.
What are the columns used in hplc?
* large columns, specifically for packing. * analytical columns, for quantitative analysis, usually accompanied by a UV-vis detector. * Narrow bore columns, for more sensitive analysis * capillary columns, very this silica columns used almost exclusively with GC mass spectroscopy. * packed bed columns. with silica beads. and may have groups attached, e.g. C18.
Searching for the sources where you can buy Silica Gel TLC Plates for Column Chromatography?
Column Chromatography is best choice for if you are looking for Silica Gel TLC Plates for Column Chromatography. For more inquiry call on 9879203377.
What is sorbsil?
It is a column chromatography grade silica gel.
Are Adsorption and partition chromatography types of column chromatography?
Yes,both can performed in columns.
Why do open tubular columns provide greater resolution than packed columns in gas chromatography?
Open tubular columns provide greater resolution than packed columns in gas chromatography because of their large retention time.
What is the difference between vacuum chromatography and flash chromatography?
Flash chromatography uses pressure (under 10 psi) to pump solvent down a column at a rate faster than gravity would provide. Vacuum chromatography uses a vacuum at the bottom of the column to pull solvent through. Both can be performed with standard glass columns, but usually vacuum chromatography is done with a silica filled vacuum funnel instead as a rough purification technique.
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
When you use c8 and when c18 column?
Both C8 and C18 columns are considered as examples of reversed phase liquid chromatography (RP). The stationary phase here is seen as a thin film of non-polar liquid phase that has been designed to be chemically anchored to an inert material (Silica gel particles). The non-polar layer is chemically linked to the silica particles surface by reaction with the polar silanol groups on the stationary phase surface and so rendering them (less polar or non-polar), The difference between the two columns will be in the length of the carbon-chain attached to the silica surface. Acoordingly C8 hplc columns have packing material composed of silica particles attached to C8 carbon units while C18 will, of course, have packing materials coated with C18 hydrophobic units. Categorically both are reversed phase but C18 columns will definitely be more "hydrophobic" rather than the C8 columns. See also the links below.
What difference between dad and uv detector in hplc?
Liquid chromatography (LC) encompasses all chromatographic techniques using liquid mobile phase, including planar chromatography (paper chromatography and thin-layer chromatography) and column chromatography (classical column chromatography, and high-performance liquid chromatography on packed and capillary columns). The term liquid chromatography is nowadays often used as a sinonim for high performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC).
Where I can buy Silica Gel TLC Plates for Column Chromatography?
you can find these items in alibaba or sigmaaldrich website.
