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A spectrometer can provide absorbance information in a number of ways.. For example transmissivity can be given as a percentage value. The absorbance is often represented on a log scale. It may be calculated by:
A = -log10(I/I0)
Since incident light intensity must be greater than detected light intensity, Absorbance technically can't be negative.
However, a spectrometer must be zeroed before each use to provide a baseline. If a material which is contaminated or otherwise inappropriate is used to zero the spectrometer, it may give a bad baseline, and thus a sample may appear to give negative absorbance. The 'blank' which was used to zero the spectrometer would have had higher absorptivity than the sample.
Another instance in which you may get negative absorption could be fluorescence. If your material is excited by light, it can emit light at a different frequency. Detecting at this frequency may produce negative absorption. Similarly, 'upconversion' by certain porphyrins may also cause emission at certain wavelengths.
What else can I help you with?
What is a negative number to the negative power?
It can be a negative or a positive number depending on the absolute value of the power.e.g.(-4) to the power of -2 gives (1/16) and(-4) to the power of -1 gives (-1/4)
Explain why the square root of 64 has a positive and a negative value?
That simply means that there is both a positive and a negative number which, when squared, gives you 64.
What is the value of negative 9?
negative nine is the value of negative nine
What does a negative add a positive equal?
If the absolute value of the negative is bigger than that of the positive, then the answer is negative. If the absolute value of the negative is the same, then zero. If the absolute value of the negative is smaller, then positive. Absolute value is the value ignoring the sign.
What is the value of -5 to the power of two?
(-5)2 =25 Because a negative number times another negative number gives a positive number and five fives are 25
Does a positive value multiplied by a negative value equal a positive?
No, the product of the multiplication of a positive and a negative value is negative.
Will a negative number to the fifth power be a positive or negative value?
The result will be a negative value.
Can an absolute value equal a negative?
An absolute value can not be negative.
A positive number minus a negative number is?
If negative value>positive value then result is -ve If positve value>negative value then result is +ve
Does a negative value multiplied by a negative value eguals a negative?
No, like signs multiply to positive, unlike to negative.
What is the opposite of the absolute value of negative 30?
The opposite is: negative (the absolute value of negative 30).
What if you divide a negative by a positive?
You will end up with a negative value. It does not matter whether the numerator or denominator is negative. If either value is negative, the answer will be negative.
