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TB PCR blood test is done for rapid detection of Tuberculosis. This has proven to be the fastest and most effective detection method.
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1)Human immunodeficiencyvirus contains an RNA as its genetic material.Between standard PCR and RT PCR techniques, which one would you employ to amplify a desired gene from this RNA virus.Explain how you would monitor disease progression and therapy?
RT PCR Test is the most accurate test while pcr test or rapid test can get you results very quickly, the results may not always be accurate.
HBV Quantitative real time PCR blood?
my HBV VIRAL LOAD BY REAL TIME PCR IS 165 MEANS
What is a PCR test used to identify?
A PCR test amplifies a single or few copies of DNA and creates potentially thousands or millions of copies. The most common reasons are for cloning, diagnosis of hereditary disease, genetic fingerprints, and analysis of genes.
What is the purpose of a PCR machine?
A PCR machine also known as a thermal cycler is a machine used to amplify segments of dna via the PCR which stands for polymerase chain reaction. PCR machines may also be used to test temperature sensitive reactions. The first step of the machine is to heat the samples to 94-96 degrees, then the temperature is lowered to 50-65 degrees, then the mixtures temperature is raised to 72 degrees to synthesize a new dna strand.
What is the defference between Real-time PCR and reverse transcriptase PCR?
Difference between real time PCR and reverse transcription PCR is as follows:- 1. Real time PCR is donated as qPCR and on the other hand reverse transcription PCR is denoted as RT-PCR. 2. In qPCR, the template used is single strand DNA strand whereas in the RT-PCR, the template used in process is single strand of RNA. 3. The real time PCR enables both quantification as well as detection of the DNA in the real time whereas the RT-PCR enables only the quantification of the RNA and it is little bit slower process then the qPCR as it first produce the cDNA from the template RNA strand and then process it in the similar fashion as the traditional PCR.
1)Human immunodeficiencyvirus contains an RNA as its genetic material.Between standard PCR and RT PCR techniques, which one would you employ to amplify a desired gene from this RNA virus.Explain how you would monitor disease progression and therapy?
RT PCR Test is the most accurate test while pcr test or rapid test can get you results very quickly, the results may not always be accurate.
HBV Quantitative real time PCR blood?
my HBV VIRAL LOAD BY REAL TIME PCR IS 165 MEANS
How will you detect the presence of casein?
With ELISA test or other allergen test like pcr or atp.
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What is a PCR test used to identify?
A PCR test amplifies a single or few copies of DNA and creates potentially thousands or millions of copies. The most common reasons are for cloning, diagnosis of hereditary disease, genetic fingerprints, and analysis of genes.
What is the difference between PCR-genital testing and STD testing?
PCR-genital testing is a specific type of test that uses polymerase chain reaction (PCR) technology to detect the presence of genetic material from pathogens in genital samples. STD testing, on the other hand, is a more general term that refers to a range of tests used to diagnose sexually transmitted infections (STIs) through various methods, including blood tests, urine tests, and swabs. PCR-genital testing is a more targeted approach that may be used as part of an STD testing regimen to detect specific pathogens in the genital area.
When is HIV RNA detected in HIV blood?
In blood, what you are checking for is viral antibodies not HIV RNA. Your body produces antibodies to antigens (e.g. HIV proteins) to combat foreign bodies.The first test for HIV is called ELISA. This is a sensitive test that will be positive for many people that are not infected. The reason for this is to capture everybody that could POSSIBLY be infected. This is done to rule out those that do not have HIV.The next test that follows ELISA is a Western Blot test. This is a specific test for HIV viral proteins. This test is to rule in those that do have HIV.If you have an acute infection of HIV, you may not have the antibodies that are produced for the tests above. It takes approximately 2 months for antibodies to show up in your blood. In this case you can do a viral load count using PCR.
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
When donating blood what is blood tested for?
Current FDA guidelines require the following screening tests be performed on all volunteer blood donations: ABO/Rh typing Unexpected antibody screen Hepatitis B core antibody Hepatitis B surface antigen Hepatitis C antibody HIV-I/II antibody Serologic Test for Syphilis HTLV-I/II antibody T. cruzi antibody (Chagas' Disease) HIV-I Nucleic Acid Test HCV Nucleic Acid Test West Nile Virus Nucleic Acid Test Bacterial contamination of platelets screen Optional Tests: Cytomegalovirus antibody Hepatitis B Nucleic Acid Test. Sources: FDA.gov AABB.org
What are the differences between conventional pcr andreal time pcr?
PCR allows amplification of DNA for a specific gene, after too many cycles of PCR the result will reach saturation, basically meaning all of the DNA has been amplified. Conventional PCR will basically tell you whether or not a gene is expressed in your sample. This can be done semi-quantitavely if the PCR is performed for a low number of cycles, ie it will tell you whether one sample expresses more of your gene of interest than another sample. The results are seen by separating the PCR products by agarose gel/ethidium bromide electrophoresis. Real-time PCR will record exactly what cycle of PCR a detectable level of amplified product became detectable, giving a far more accurately quantifiable estimation of gene expression.
How do you use a Southwell Plot to estimate the critical load and initial imperfection of a column?
The Southwell Plot method enables you to test a column in a non-destructive manner so that its buckling behaviour can be determined. If we look at the Southwell Plot equation: P/y = 1/Pcr * y + a/Pcr Here we have: P = Applied load y = mid-height displacement Pcr = Critical buckling load a = initial mid-height deformation (or imperfection as you called it) Using test data, you can plot a graph with P/y along the y-axis and y along the x-axis. This will give you a slope of 1/Pcr and an intercept at y=0 of a/Pcr. From there the value of 'Pcr' and 'a' can be determined. It is interesting to see how the slope of this curve is often very linear, especially for elastic buckling cases. It is for this reason that you do not need to test a column to failure.
What is the use of dNTP?
The use of dNTP is PCR and multiplex PCR
