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Nested PCR is a variation of regular PCR that involves two rounds of amplification. It is often used when the target DNA is present in low concentrations. Nested PCR can increase the sensitivity and specificity of the test compared to regular PCR. Regular PCR, on the other hand, involves a single round of amplification and is commonly used for routine DNA amplification. Nested PCR is advantageous in detecting low abundance targets, while regular PCR is more suitable for general DNA amplification purposes.

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What did you learn from this lab?

From this lab, I learned about the process of DNA extraction, PCR amplification, gel electrophoresis, and the importance of proper controls and techniques in molecular biology experiments. I also gained a deeper understanding of how these techniques are used in research and diagnostic applications.


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How does molecular genetics add to your understanding of genes?

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Differences between living and non living on the molecular basis?

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