The enzyme polymerase is functional in water medium, that's why water is needed. Do understand that biological polymerization reactions as in nucleic acids and other molecules, are condensation reaction (dehydration synthesis) where water is liberated.
Because the neutron economics are against it, a critical reactor and a chain reaction would not be possible. Ordinary water absorbs too many neutrons. For natural uranium you must use graphite or heavy water for the moderator.
A reactant is an "ingredient" in a chemical reaction; it is a substance you have at the beginning of a reaction. A product is what gets produced in a chemical reaction; it is a new substance you did not have before. Reactants yield products.
yes you can. Most acetates are water soluble so no reaction is likely to happen, except of course dissolution of alum.
There are many ways to clean a stainless steel chain. But in order to correctly clean it, the best way is to use bleach and add water to the bleach to clean it.
(aq) It means aqueous. Example: NaCl(aq)
Polymerase chain reaction
Polymerase chain reaction
cloning a DNA library. genetic amplification. the use of reverse transcriptase. the action of DNA polymerase
In 1984, Dr. Mullis developed the use of the Polymerase Chain Reaction (PCR) technique to replicate DNA. For this, he was the co-recipient of the 1993 Nobel Prize in Chemistry.
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
The term pcr in biology stands for polymerase chain reaction. It stands for a process that biologists use in DNA sequencing, allowing them to make DNA copies more efficiently.
Run gel electrophoresis :: Apex
To ramp up the DNA into a large enough amount to work with you would use polymerase chain reaction technique. PCR. By using the enzymes found in certain extremeophiles one alternately heats and cools the DNA solution with this extremeophile polymerase included and it ramps up the amount of DNA so you have a useful bit of DNA for insertion into a cloning vector.
DNA polymerase-polymerase chain reaction to amplify sections of DNA reverse transcriptase-production of cDNA from mRNA DNA ligase-cutting DNA, creating sticky ends of restriction fragments restriction enzyme-analysis of RFLPs electrophoresis-separation of DNA fragments
Polymerase Chain Reaction, also known as PCR, can be used to rapidly make multiple copies of a gene using a primer.Another method is to use a plasmid vector to carry, store and multiply a gene in a microbial cell, such as E. coli.
Polymerase Chain Reaction, also known as PCR, can be used to rapidly make multiple copies of a gene using a primer.Another method is to use a plasmid vector to carry, store and multiply a gene in a microbial cell, such as E. coli.
Mammalian polymerase is heat labile. Which means, it denatures (or breaks up into fragments) at higher temperatures. Since PCR is a reaction what requires a high temperature for the DNA strands to denature, it would be more efficient to use a polymerase that could function at higher temperatures.