The enzyme polymerase is functional in water medium, that's why water is needed. Do understand that biological polymerization reactions as in nucleic acids and other molecules, are condensation reaction (dehydration synthesis) where water is liberated.
Hydrolysis is the chemical reaction that involves the use of water to break down biomolecules.
Because the neutron economics are against it, a critical reactor and a chain reaction would not be possible. Ordinary water absorbs too many neutrons. For natural uranium you must use graphite or heavy water for the moderator.
Heavy water, which contains deuterium instead of regular hydrogen, is used as a moderator in nuclear reactors because it slows down neutrons more efficiently than regular water. This helps maintain a sustained nuclear reaction by increasing the likelihood of neutron interactions with fuel nuclei. Additionally, heavy water is less prone to absorbing neutrons, which can help sustain the nuclear chain reaction.
Light water reactors use enriched fuel because natural uranium typically does not have a high enough concentration of the fissile isotope uranium-235 to sustain a nuclear chain reaction. Enrichment increases the proportion of uranium-235 in the fuel to enable sustained fission reactions.
The most important endothermic reaction is photosynthesis, where plants use sunlight to convert carbon dioxide and water into glucose and oxygen. This process is essential for producing oxygen, removing carbon dioxide from the atmosphere, and providing energy for plants and other organisms in the food chain.
No, PCR (polymerase chain reaction) uses DNA primers, not RNA primers, in its process.
Polymerase chain reaction
Yes, Vent polymerase is a thermostable enzyme. It is derived from the Thermococcus species and is able to withstand high temperatures, making it suitable for use in applications that require high-temperature conditions such as polymerase chain reaction (PCR).
Polymerase chain reaction (PCR) is a widely used technique that allows scientists to amplify specific DNA sequences in a test tube. This process involves repeatedly heating and cooling the DNA to facilitate replication. Another method is isothermal amplification, which amplifies DNA at a constant temperature using enzymes like Bst polymerase.
In 1984, Dr. Mullis developed the use of the Polymerase Chain Reaction (PCR) technique to replicate DNA. For this, he was the co-recipient of the 1993 Nobel Prize in Chemistry.
a process called polymerase chain reaction (PCR), which amplifies a specific region of DNA in vitro. During PCR, DNA is denatured, primers bind to the target sequence, and DNA polymerase synthesizes new DNA strands, resulting in multiple copies of the target region. This technique is widely used in research, diagnostics, and forensic analysis.
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
True. The process of polymerase chain reaction (PCR) is similar to the process of DNA replication that occurs in cells. Both processes involve the use of DNA polymerase enzyme to synthesize new strands of DNA using a template strand. However, PCR occurs in a controlled laboratory setting and amplifies specific target DNA sequences, while DNA replication is a natural cellular process that duplicates the entire genome.
PCR stands for polymerase chain reaction, a method widely used in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA sequence.
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To ramp up the DNA into a large enough amount to work with you would use polymerase chain reaction technique. PCR. By using the enzymes found in certain extremeophiles one alternately heats and cools the DNA solution with this extremeophile polymerase included and it ramps up the amount of DNA so you have a useful bit of DNA for insertion into a cloning vector.
DNA polymerase-polymerase chain reaction to amplify sections of DNA reverse transcriptase-production of cDNA from mRNA DNA ligase-cutting DNA, creating sticky ends of restriction fragments restriction enzyme-analysis of RFLPs electrophoresis-separation of DNA fragments