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The older format, AT, will power up without a motherboard. ATX form factor needs motherboard connection to power up. However, for about $15 you can purchase a PSU tester that plugs onto the psu to allow it to power up without the motherboard. Try: dalco.com, or FRY's etc.

Actually, ATX will start without a motherboard, or a 'PSU tester'. The ATX motherboard power connector will have 1 green wire, and several wires of other colours. Find a bit of lose wire, it doesnt need to be very long. Make sure both ends of the wire are stripped. Push one end of the lose wire into the 'hole' that the green wire connects to, then push the other end into any 'hole' that has a black wire leading to it . The PSU should start, and stop when you remove the lose wire. == ==

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Q: Will a computer's power source function without a connection to the motherboard?
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