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No, of course it is possible that one could start out with an already diluted sample in the first step. So then perhaps in the third sector some isolated colonies may form, because the amount of bacteria have been diluted enough.

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14y ago
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8y ago

Not always. Depending on the amount you started with you may get isolated colonies in the third sector.

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Q: Will isolated colonies of bacteria always be in the fourth sector on the streak plate?
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