Will lenses from your Canon 1000 EOS film camera work on any Canon EOS digital?

The short answer is "Yes." The long answer is: All Canon EOS cameras use Canon EF (electro-focus) lenses. They can also use independently made lenses with EF type camera mounts. All Canon DSLR (digital single lens reflex) cameras can use all Canon EF (or independent manufacturer equivalent) lenses made for film or digital cameras. Most Canon DSLR's have an APS-C sensor that measures 22.2 x 14.8mm. (This is smaller than the 36 x 24mm image size on 35mm film.) In 2003, Canon introduced EF-S lenses that were optimized for the APS-C sensor size. The EF-S lenses will only fit on Canon 20D, 30D, 40D, and 50D cameras (as of 2008) and all Canon Digital Rebel cameras.