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When does a spray of diphenylamine reagent on TLC gives blue coloration?
Diphenylamine is a reagent for DNA.
What are the methods for estimation of DNA?
diphenylamine reagent
Does water react with diphenylamine?
One major difference between DNA and RNA is their sugar: DNA contains deoxyribose, whereas RNA contains ribose. This is what isdiphenylamine testfor deoxyribose for. In this process, the reaction between the Dische reagent and 2-deoxypentose results in the development of a blue color. The reaction depends on the conversion of the pentose to w-hydroxylaevulinic aldehyde which then reacts with diphenylamine to give a a blue colored complex. The intensity of the blue color is proportional to the concentration of DNA. Dische reagent does not react with the ribose sugar in RNA and does not form a blue-colored complex.Dische diphenylamine test-- A chemical test utilized to detect the presence of DNA in a substance.Mudit Mishra
What function does DNA polymerase have to enable it to detect errors and correct them?
proofreading
What materials do scientists typically use when extracting DNA?
phenol,chloroform,isoamyl alcohol,ethanol,CTAB reagent,Na acetate etc.. but nowadays, they use kits for any kind of DNA isolation, which makes their job easier.
When does a spray of diphenylamine reagent on TLC gives blue coloration?
Diphenylamine is a reagent for DNA.
What are the methods for estimation of DNA?
diphenylamine reagent
Dana was studying viral DNA Which of the following would she NOT detect?
ribose
What are the factors that can affect the transfection efficiency and how?
seeding density of the cell quality of transfecting dna ratio of dna to transfectant reagent
Does water react with diphenylamine?
One major difference between DNA and RNA is their sugar: DNA contains deoxyribose, whereas RNA contains ribose. This is what isdiphenylamine testfor deoxyribose for. In this process, the reaction between the Dische reagent and 2-deoxypentose results in the development of a blue color. The reaction depends on the conversion of the pentose to w-hydroxylaevulinic aldehyde which then reacts with diphenylamine to give a a blue colored complex. The intensity of the blue color is proportional to the concentration of DNA. Dische reagent does not react with the ribose sugar in RNA and does not form a blue-colored complex.Dische diphenylamine test-- A chemical test utilized to detect the presence of DNA in a substance.Mudit Mishra
How can a person detect the dna of a dog?
To detect the DNA of a dog, it would require lots of scientific and medical equipment. Taking a blood sample would be a good place to start, but without the correct knowledge of how to use the blood sample, it is likely to be a waste of time and a painful experience for the dog.
What function does DNA polymerase have to enable it to detect errors and correct them?
proofreading
How can PCR help to detect very low amounts of DNA?
it do so by amplification of the small DNA fragment ( with the help of promoters and enzymes)
What is a non example of amino acid?
Non-protein nitrogen (or NPN) is a term used in animal nutrition to refer collectively to components such as urea, biuret, and ammonia, which are not proteins but can be converted into proteins by microbesin the ruminant stomach
What materials do scientists typically use when extracting DNA?
phenol,chloroform,isoamyl alcohol,ethanol,CTAB reagent,Na acetate etc.. but nowadays, they use kits for any kind of DNA isolation, which makes their job easier.
A special enzyne scans the DNA helix in search of improper nitrogen base pairings how would the enzyne be able to detect incorrect nitrogen base pairings?
stupid science stuff
Does holy ghost mean DNA?
The concept of the Holy Ghost was introduced into Christianity in the early middle ages, centuries before the discovery of DNA, so clearly it is not intended to mean DNA. It has no connection to DNA that I can detect. Forget what they instilled in you at school, THINK
