ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood.
Alternative NamesEnzyme-linked immunoassay; EIA
How the test is performedBlood is typically drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.
Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm.
Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.
In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding.
The sample is sent to a laboratory where the targeted antibody (or antigen) is linked to an enzyme. If the target substance is in the sample, the test solution turns a different color.
How to prepare for the testNo special preparation is needed.
How the test will feelWhen the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.
Why the test is performedThis test is often used to see if you have been exposed to viruses or other infectious substances. It is frequently used to screen for present or past infections.
Normal ValuesNormal values depend on the type of substance being identified. Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.
What abnormal results meanAbnormal values depend on the type of substance being identified. In some people, a positive result may be normal.
What the risks areVeins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.
Other risks associated with having blood drawn are slight but may include:
Ashihara Y, Kasahara Y, Nakamura RM. Immunoassay and immunochemistry. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 21st ed. Philadelphia, Pa: Saunders Elsevier; 2006:chap 43.
ELISA stands for enzyme-linked immuno assay. It is a commonly used laboratory test to detect antibodies in the blood.
Alternative NamesEnzyme-linked immuno assay; EIA
How the test is performedBlood is typically drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.
Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm.
Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.
In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding.
The sample is sent to a laboratory where the targeted antibody (or antigen) is linked to an enzyme. If the target substance is in the sample, the test solution turns a different color.
How to prepare for the testNo special preparation is needed.
How the test will feelWhen the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.
Why the test is performedThis test is often used to see if you have been exposed to viruses or other substances that cause infection. It is often used to screen for current or past infections.
Normal ValuesNormal values depend on the type of substance being identified. Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.
The examples above show the common measurements for results for these tests. Some laboratories use different measurements or may test different specimens.
What abnormal results meanAbnormal values depend on the type of substance being identified. In some people, a positive result may be normal.
What the risks areVeins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.
Other risks associated with having blood drawn are slight but may include:
Ashihara Y, Kasahara Y, Nakamura RM. Immunoassay and immunochemistry. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 21st ed. Philadelphia, Pa: Saunders Elsevier; 2006:chap 43.
Reviewed ByReview Date: 10/30/2010
Linda Vorvick, MD, Medical Director, MEDEX Northwest Division of Physician Assistant Studies, University of Washington School of Medicine. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc.
Not including the antigen will prevent the primary antibody from binding to it which will disrupt the results of the ELISA. Not including the primary antibody will prevent the secondary antibody from binding it, which will again negatively affect the results of the ELISA. All components are necessary to get an accurate ELISA.
In "The Chrysanthemums" Elisa is heading into town for dinner when she spies a speck in the road. She believes that the speck is actually her bunch of chrysanthemums.
There needs to be a conversion of the substrate for the chemical reaction to provide an H+ ion for the color change needed for ELISA. The temperature essentially lowers the activation energy to allow for this reaction to proceed.
like ELISA, PAT is also a test of AIDS It is a test for electrical safety it stands for Portable Appliance Testing
So that when the substrate is added, the reaction between the enzyme and the substrate will cause a change in color
Elisa's was created in 2009.
Elisa Jordana's birth name is Elisa Ann Schwartz.
Elisa Aldridge's birth name is Elisa Deanne Aldridge.
Gillian Elisa's birth name is Gillian Elisa Thomas.
Elisa Fernandes's birth name is Elisa Fernandes Leite.
Elisa Briganti's birth name is Elisa Livia Briganti.
Elisa Veek's birth name is Elisa Vignochi Veeck.
Elisa Bridges's birth name is Elisa Rebeca Bridges.
Elisa Salo's birth name is Elisa Sini Maria Salo.
Elisa Lamotte's birth name is Andre Elisa Amlie Lamotte.
The cast of Elisa - 2012 includes: Anna Carvalho as Elisa
Elisa Cegani's birth name is Elisa Angela Maria Cegani.