Osmotic fragility is a test to detect whether red blood cells are more likely to break down.
How the test is performedBlood is typically drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.
Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.
In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding.
In the laboratory, red blood cells are tested with a solution that makes them swell, in order to determine how fragile they are.
How to prepare for the testNo special preparation is necessary for this test.
How the test will feelWhen the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.
Why the test is performedThis test is performed to detect hereditary spherocytosisand thalassemia. Hereditary spherocytosis makes red blood cells more fragile than normal. Some red blood cells in patients with thalassemia are more fragile than normal, but a larger number are less fragile than normal.
Normal ValuesA negative test is normal.
Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.
What abnormal results meanVeins and arteries vary in size from one patient to another, and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.
Other risks associated with having blood drawn are slight but may include:
Golan DE. Hemolytic anemias: red cell membrane and metabolic defects. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier; 2007: chap 165.
Gallagher PG, Jarolim P. Red Blood Cell Membrane Disorders. In: Hoffman R, Benz EJ, Shattil SS, et al, eds. Hematology: Basic Principles and Practice. 5th ed. Philadelphia, Pa: Elsevier Churchill Livingstone; 2008:chap 46.
Osmotic fragility is a test to detect whether red blood cells are more likely to break down.
How the test is performedA blood sample is needed. For information on how this is done, see: Venipuncture
In the laboratory, red blood cells are tested with a solution that makes them swell, in order to determine how fragile they are.
How to prepare for the testNo special preparation is necessary for this test.
How the test will feelWhen the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.
Why the test is performedThis test is performed to detect hereditary spherocytosisand thalassemia. Hereditary spherocytosis makes red blood cells more fragile than normal. Some red blood cells in patients with thalassemia are more fragile than normal, but a larger number are less fragile than normal.
Normal ValuesA negative test is normal.
Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your doctor about the meaning of your specific test results.
What abnormal results meanVeins and arteries vary in size from one patient to another, and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.
Other risks associated with having blood drawn are slight but may include:
Golan DE. Hemolytic anemias: red cell membrane and metabolic defects. In: Goldman L, Ausiello D, eds. Cecil Medicine. 24th ed. Philadelphia, Pa: Saunders Elsevier; 20011:chap 164.
Gallagher PG, Jarolim P. Red blood cell membrane disorders. In: Hoffman R, Benz EJ, Shattil SS, et al., eds. Hematology: Basic Principles and Practice. 5th ed. Philadelphia, Pa: Elsevier Churchill Livingstone; 2008:chap 46.
Reviewed ByReview Date: 02/08/2012
Todd Gersten, MD, Hematology/Oncology, Palm Beach Cancer Institute, West Palm Beach, FL. Review provided by VeriMed Healthcare Network. Also reviewed by Linda J. Vorvick, MD, Medical Director and Director of Didactic Curriculum, MEDEX Northwest Division of Physician Assistant Studies, Department of Family Medicine, UW Medicine, School of Medicine, University of Washington; David Zieve, MD, MHA, Medical Director, A.D.A.M. Health Solutions, Ebix, Inc.
-Acidified glycerol Lysis test - the Pink Test - NESTROFT ( naked eye single tube red cell osmotic fragility test ) :) - i tried to search this and i guess this would help other medtech students and to anyone related to it :) by: Peter Paul M. Pastor heheee!! XD
The osmotic saline laxative work by causing an osmotic effect. This osmotic effect takes place primarily within the small intestines. This causes, eventually, a movement of the bowels.
osmotic- pressure exerted by large molecule oncotic- presure exserted by proteins
osmotic
Osmotic pressure
Osmotic fragility usually (unless otherwise mentioned) refers to the ease with which Red Blood Cells (RBCs) undergo lysis in a hypotonic solution. Before knowing what 'low osmotic fragility' means, one should know the concept of osmotic fragility. Different parameters, some of them related to the RBC and some of the extracellular environment have an effect on osmotic fragility. Low osmotic fragility means that the RBCs have a resistance against undergoing lysis when suspended in solutions which are hypotonic. They undergo lysis only if the solution is extremely hypotonic. In contrast, high osmotic fragility refers to the tendency of the RBCs to lyse even if the solution in which they are suspended in is mildly hypotonic. As an example for low osmotic fragility would be thalassemia. And as an example for high osmotic fragility, spherocytosis (a hereditary condition where the RBCs lose their usual biconcave structure and become spherical) causes increased osmotic fragility. i.e, It very easily lyses even at solutions which are mildly hypotonic... p.s Another interesting point to note is that in sickle cell anemia, the RBCs show increased mechanical fragility but decreased osmotic fragility. Elucidated by the fact that post-splenectomy the RBC lifespan increases.
the cell respon differently to different solution
The cell responds differently to different solutions
-Acidified glycerol Lysis test - the Pink Test - NESTROFT ( naked eye single tube red cell osmotic fragility test ) :) - i tried to search this and i guess this would help other medtech students and to anyone related to it :) by: Peter Paul M. Pastor heheee!! XD
Osmotic alterations can be defined as the fragility of the red blood cells in hypo and hyperthyroid patients. Changes in the concentration of the thyroid hormone can affect Na+K+ATPase number and activity and the phpspholipid composition of the cell membranes.
the change of osmotic pressure over time
Pertaining to, or having the property of, osmose; as, osmotic force.
The osmotic coefficient of sodium chloride is 0,93.
The osmotic saline laxative work by causing an osmotic effect. This osmotic effect takes place primarily within the small intestines. This causes, eventually, a movement of the bowels.
Cytolysis or Osmotic-lysis
TDS increases the osmotic pressure. From the formula, Osmotic pressure = CRT. C as the concentration in mol / L; R = gas constant; T = temperature. The higher the concentration of the solids, the higher is its osmotic pressure.
The osmotic pressure is 24,44 at.