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What are the conclusions from testing the Biuret reagent for protein?
The reagent used in the Biuret Test is a solution of copper sulfate (CuSO4) and potassium hydroxide (KOH). The KOH is there to raise the pH of the solution to alkaline levels; the crucial component is the copper (II) ion from the CuSO4. When peptide bonds are present in this alkaline solution, the copper (II) ions will form a coordination complex with four nitrogen atoms involved in peptide bonds. Copper Sulfate solution is a blue colour, but when the copper (II) ions are coordinated with the nitrogen atoms of these peptide bonds, the colour of the solution changes from blue to violet. This colour change is dependent on the number of peptide bonds in the solution, so the more protein, the more intense the change. When the peptides are very short, the solution turns a pink colour, rather than violet.
What is a pH indicators?
A pH indicator is a halochromic chemical compound that is added in small amounts to a solution so that the pH (acidity or alkalinity) of the solution can be determined easily. Normally, the indicator causes the colour of the solution to change depending on the pH.
How do you prepare kedd's reagent?
card. + Kedde's reagent (3,5- dinitrobenzoic acid + NaOH → violet colour.
What colour does iodine solution turn red litmus paper?
The color not change.
What is the colour change in Protein when the Bluret test is performed?
When being tested with biuret reagent, protein turns a blue to lilac color. However, starches when tested do change to a blackish blue color.
What colour is obtained on adding KSCN reagent to ferric ion solution?
Intense Red Colour
What does the Benedict's solution test show?
A colour change from the original blue to a brick red colour, although various intermediary colours can also form depending on the concentration of the glucose.if reducing suger is present the colour of the solution will turn to brick red and that will be a positive test.my answer: Benedict's reagent contains copper sulphate, since glucose is educing sugar it will turn Cy +2 into a reagent , to cu +1.
What are the conclusions from testing the Biuret reagent for protein?
The reagent used in the Biuret Test is a solution of copper sulfate (CuSO4) and potassium hydroxide (KOH). The KOH is there to raise the pH of the solution to alkaline levels; the crucial component is the copper (II) ion from the CuSO4. When peptide bonds are present in this alkaline solution, the copper (II) ions will form a coordination complex with four nitrogen atoms involved in peptide bonds. Copper Sulfate solution is a blue colour, but when the copper (II) ions are coordinated with the nitrogen atoms of these peptide bonds, the colour of the solution changes from blue to violet. This colour change is dependent on the number of peptide bonds in the solution, so the more protein, the more intense the change. When the peptides are very short, the solution turns a pink colour, rather than violet.
Do magnetic effect of electric current causes a change in colour in a solution through which electric current is passed?
i don't believe it does
What's the difference in indicator and reagent?
Indicator will changes the colour according to H+ ion present in a solution. Reagent : consist of electrophile and nucleophile. It help in the attachment of nucleophile to the electrophile and electrophile to the nucleophile. Mirza
What is a indicators?
A pH indicator is a halochromic chemical compound that is added in small amounts to a solution so that the pH (acidity or alkalinity) of the solution can be determined easily. Normally, the indicator causes the colour of the solution to change depending on the pH.
What is the result of the seliwanoff test if the solution contains both fructsoe and glucose?
the glucose would remain yellow which is the initial colour of the seliwanoff's reagent. the fructose however being a ketose forms a furfural because of the HCL present in the seliwanoff reagent and reacts with the resorcinol to give a red cherry or pink colour.
What is a pH indicators?
A pH indicator is a halochromic chemical compound that is added in small amounts to a solution so that the pH (acidity or alkalinity) of the solution can be determined easily. Normally, the indicator causes the colour of the solution to change depending on the pH.
What is the function of alkaline solution in Biuret's test?
Alkaline levels are essential for the solution to change colour. Without alkaline levels, the colour of the solution would not be detectable.
What colour of sugar solution on litmus paper?
Litmus does not change color in sugar solution.
How do you prepare kedd's reagent?
card. + Kedde's reagent (3,5- dinitrobenzoic acid + NaOH → violet colour.
What causes the change of a litmus paper color?
Litmus contains organic chemicals that have a particular colour (some kind of red) when protonated - that is, in acidic conditions - but a different colour (blue) when in basic conditions or when they have donated protons. We see this colour change and can identify whether a solution is nonacidic or nonbasic by using one of these strips.
