The duration of autoclaving typically ranges from 15 to 60 minutes, depending on the type of materials being sterilized and the specific protocol being used. Standard cycles often involve a 15-30 minute exposure at 121°C (250°F) under pressure. However, thicker or more densely packed items may require longer times to ensure complete sterilization. Additionally, time for heating up and cooling down the autoclave should also be considered.
Instruments typically remain sterile for about 30 days after autoclaving, as long as they are stored properly in a clean and dry environment.
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it is unacceptable
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Autoclaving
autoclaving
Autoclaving uses high pressure and temperature to sterilize instruments and materials, which is more effective at killing all types of microorganisms, including spores, compared to boiling which may not reach a high enough temperature. Autoclaving also achieves sterilization more quickly than boiling, making it a more efficient method for healthcare settings.
Boiling cannot sterilize scissors but heating at temperatures above the boiling point of water in an autoclaving (121oC) would sterilize scissors after 15-20 minutes.
Autoclaving is a method of sterilizing equipment by the use of pressurized high temperature steam. It may be used to sterilize surgical equipment for reuse or for sterilizing bottles prior to filling them with beer, soda pop, or other beverages.
Bovine serum albumin (BSA) can undergo some degradation after autoclaving due to the high temperatures and pressures involved in the process. While autoclaving effectively sterilizes solutions, it can denature proteins, including BSA, leading to loss of functionality. If BSA is required for specific applications, it is generally recommended to use lower temperature methods for sterilization, such as filtration, to preserve its structural integrity and activity.
Autoclaving is used to kill all micro-organisms that are in the culture. Autoclaving is usually carried out at 121 deg C with steam under 2 atmospheres of pressure. A clean culture free of all micro-organisms is essential as you only want the organism of intrest to grow.
Assuming you mean commercially packaged.After it is jarred or canned, it is pressure cooked under high pressure long enough to sterilize it completely- just like autoclaving equipment in the hospital.