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Biological isolation occurs when an individual or in most cases a population of individuals do not have contact with other species that they are capable of breeding with or infecting. This process can be man made or natural. In man made we isolate organisms in a sterile environment such as a level 4 Biology lab, special greenhouses, and on special farms. Natural isolation can occur when a population of organisms travel to islands from the mainland and no longer breed with organisms from the mainland. Occasionally natural disasters such as earthquakes, mud slides, etc can force a population into a small portion of its previous environment separating the population into different areas. These populations can then evolve separately.

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