When using a high power objective, you should use the fine adjustment knob. This knob allows for precise focusing and helps to avoid damaging the slide or the objective lens due to the closer working distance. The coarse adjustment knob should be reserved for lower power objectives to prevent any potential contact between the lens and the specimen.
For low power you can use the coarse adjustment and fine adjustment for high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
The course-adjustment knob moves the stage up and down quickly, and using it with a high-power objective can potentially damage the objective or slide due to a rapid change in focus. It is best to use the fine-adjustment knob when using a high-power lens for precise focusing.
use the fine adjustment knob to get a better view of what you are looking at.For low power you can use the coarse adjustment and fine adjustmentfor high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
use the fine adjustment knob to get a better view of what you are looking at.For low power you can use the coarse adjustment and fine adjustmentfor high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
The coarse focus knob should be used with the low power objective but not with the high power objective. The point of using the coarse focus knob is to bring the specimen into general focus before fine-tuning with the high power objective, which requires more precise adjustments.
The fine adjustment knob should be used with high power magnification because it allows for smaller, more precise movements of the objective lens. This helps to prevent damage to the slide and objective lens, as well as minimize the risk of crashing the objective lens into the slide.
The coarse adjustment knob on a microscope should never be used with the high-power objective lens. This can cause damage to both the lens and the slide being viewed. Instead, it is best used with the low-power or scanning objective lens for initial focusing.
When focusing on a specimen under high power, you should use the fine adjustment knob. The coarse adjustment knob is typically used only under low power to prevent damage to the slide or objective lens. The fine adjustment allows for precise focusing, which is crucial at high magnifications to achieve a clear and sharp image of the specimen. Always ensure that you start with the lowest power objective before switching to high power for safety.
First of all, one should never call it high power, it is morecommonly called the "high objective", yet that is not what this question is asking. To answer the question: You use the fine adjustment knob. This knob should be located near the coarse adjustment knob, on the opposite side of the microscope (at the same height as the coarse adjustment knob), or even as a separate knob protruding from the coarse adjustment knob. The fine adjustment knob is smaller in size. You should never, ever use the coarse adjustment knob under the high objective, you could scratch the microscope slide, cover slip, high objective lens, or in a worst case scenario break the high objective lens.
When working with a microscope you should always take procauctions but one question I am asked a lot is When working with a microscope do you open or close the diaphram when working with the medium- and high-power objective nobs? The answer is you keep it open if you don't you won't be abler to see any thing.
The fine adjustment knob should be used only with the high power objective (HPO) because it allows for precise focusing on small details at higher magnifications. When using HPO, the working distance is significantly reduced, and using the coarse adjustment knob could lead to crashing the objective lens into the slide, potentially damaging both the slide and the lens. The fine adjustment knob provides the delicate control needed to achieve clear images without risk of contact.
The fine adjustment knob should be used only with the high-power objective (HPO) because it allows for precise focusing at high magnifications. Using the fine adjustment knob with lower-power objectives can result in the objective lens hitting the slide, potentially damaging both the lens and the slide.