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Why do you use the same restriction enzyme when you splice together two separate things?
Using the same restriction enzyme when splicing DNA into plasmids, etc., is effective as restriction enzymes are site-specific. Therefore, the spliced DNA will be able to complementary base pair with the ends of the spliced plasmid due to the identical recognition sites. Since the two molecules have the same sticky ends, they will be able to fit together.
What else can I help you with?
What was the first practical use of a restriction enzyme in production of what?
The first practical use of a restriction enzyme was in the production of recombinant DNA in the early 1970s. Scientists used the restriction enzyme EcoRI to cut DNA at specific sequences, allowing them to splice together DNA fragments from different sources. This innovation enabled the development of genetically modified organisms and the production of insulin and other therapeutic proteins. The ability to manipulate DNA in this way revolutionized molecular biology and biotechnology.
How do you make a single wrapped splice?
To make a single wrapped splice in rope or fishing lines, first overlap the two ends you want to splice and tape them together. Then, use a separate piece of line to wrap tightly around the splice, tucking the ends underneath the wraps for security. Trim any excess and ensure the splice is tight.
What are the rules of comma splice?
A comma splice joins two independent clauses - a no-no. An independent clause is one that can stand alone as a sentence. When two independent clauses are next to each other, you have only two choices: you can either join them, or you can separate them. you can join them with a coordinator word, such as but, if, therefore. You can separate them with end-point punctuation (;, - )
Enzymes used to cut DNA molecules in recombinant DNA researsh are?
Restriction enzymes are used to cut DNA molecules in recombinant DNA research. These enzymes recognize specific DNA sequences and cleave the DNA at those sites, allowing scientists to splice DNA fragments from different sources together to create recombinant DNA molecules.
Believing that it encourages people to reject class divisions you practically invented montage with a scene in the Battleship Potemkin in which you splice together 155 separate shots into four minutes?
Sergei Eisenstein
What is a Y- sPLICE?
A splice is usually two rope ends joined together to form a longer rope. A Y splice is three rope ends are joined together to form a Y shape. The same can be said for connecting three electrical or component cables.
What is a basic technique for gene transfer?
Firstly, the plasmid is removed from the bacteria where it is cut open by restriction enzymes. The desired DNA is then extracted from the donor, which is then cut open with the same restriction enzyme. This results in sticky ends and the two pieces of DNA from the two organisms can hence be mixed with complementary base sequences. Ligase is then used to splice the pieces of DNA to form recombinant plasmids, which is then inserted into host cells.
How do you conect the wires to the electric windows the wires have been cut?
Match the wires by their color codes and splice them together with splice connectors and a crimping tool.
How would you see if a sentence had a comma splice?
If a sentence consists of two independent clauses with a comma between them, it is a comma splice. That is, the part before the comma can stand on its own as a sentence, and the part after the comma can also stand on its own as a sentence, then it is a comma splice. If there is no punctuation there instead of a comma, it is known as a run-on sentence.
What is electrical splice?
The joining together of two or more conductors in an electric circuit.
What is the length of a log cabin?
As long as you want, provided you splice logs together.
How are splice sites recognized?
Splice sites in mRNA are recognized by the spliceosome, a complex of proteins and small nuclear ribonucleoproteins (snRNPs). The spliceosome identifies the consensus sequences at the splice sites, including the 5' splice site, the branchpoint site, and the 3' splice site. The spliceosome machinery then removes introns and ligates together exons to produce the mature mRNA transcript.
