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The count rate of carbon-14 (subtracting background radiation) from a fresh sample of the same substance you are testing so that one can compare the count rate of the old artifact. The half-life of Carbon-14 (5730 years) must also be known.

You must know the background radiation to find the actual radiation being emitted from the artifact, the half-life of Carbon-14 and the total number of carbon atoms in the artifact for comparing ratios. The mass of nonradioactive carbon atoms is also needed.

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