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YES they do. Its disgraceful.

It depends on the test. Everyone complains about the Draize test, which is the one where they hold open rabbits' eyes and drip chemicals in them. The one that's worse is the LD50 test. This determines the "acute toxicity" of a substance--LD50 means the dose that kills half the animals who receive it. They can do a skin contact LD50, an oral LD50, an inhalation LD50 (more correctly LC50--inhaled chemicals are rated in parts per million in air) or an injection LD50. If you are doing an oral LD50 on antifreeze, you squirt a measured dose of antifreeze down the throats of 100 animals--anything from mice to dogs are used. You then count the number of animals that die over the next 14 days. At the end you euthanize the ones who are still alive. If 50 or more of the animals died from antifreeze, you record the dose as the LD50 and you're done. If fewer than 50 die, you repeat the test with a new batch of animals and a higher dose. Antifreeze kills by destroying the kidneys, and there are even worse chemicals than that--imagine having to run an inhalation LC50 on nerve gas!

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