blood samples were centrifuged at 7000g for 10 minutes and separated sera were frozen at -20"C until used for Liszt assay. Lysozyme level in blood samples was determined by the turbidimetric assay according to the method described by Ellis (1990) with slight modifications. Briefly, aliquots (1.75 ml) of
Micrococcus lysodeikticus suspension (Sigma) (0.375 mg/ml, 0.05 M sodium phosphate buffer, pH 6.2) was mixed with 250L of each sample and the
optical density was measured after 15 and 180 seconds by spectrophotometer (Biophotometer Eppendorf) at 670 nm wavelength. PBS was used as the blank and results were expressed in amounts of lysozyme (g) per one milligram of sample calibrated using a standard cure determined with hens egg white lysozyme (Sigma) in PBS.
Yes, lysozyme is a protein.
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Serum Osmolarity = 2 [Na] + [Glucose] + [BUN] (if all in mmol/L) OR Serum Osmolarity = 2 [Na] + [Glucose]/18 + [BUN]/2.8 (if glucose and BUN are in mg/dl)
increased serum transaminase activity is seen in liver dysfunction. greater activity of GOT over GPT is typical of myocardial infarction.
Paneth cellsIn addition to defensins, Paneth cells secrete lysozyme[6], tumor necrosis factor-alpha[6] , and phospholipase A2.[citation needed] Lysozyme and phospholipase A2 both have clear antimicrobial activity. This battery of secretory molecules gives Paneth cells a potent arsenal against a broad spectrum of agents, including bacteria, fungi and even some enveloped viruses.
basically...
The antimicrobial enzyme found in tears is called lysozyme. It helps to protect the eyes from bacterial infections by breaking down the cell walls of certain bacteria.
Schlichter, Jakub G., U.S. internist, 1912-.Schlichter test - for serum bactericidal activity. A measure of the killing capacity of the patient's serum or body fluid against the patient's infecting organism.
practical capacity
Saliva contains lysozyme, which is an antibacterial enzyme that helps protect the mouth from bacterial infections.
The normal level of lysozyme in saliva ranges from 1 to 5 mg/mL. Lysozyme is an enzyme that helps protect against bacterial infections by breaking down bacterial cell walls.
Lysozyme and mucous