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As I have been instructed by Toyota,

  1. located the bottom radiator hose,
  2. follow it to a connection to a metal tube that runs to the left side, rear of the engine,
  3. At the end of the tube, in the engine block, is the thermostat.

I'll be changing mine this weekend and I will give additional info, if needed, after the replacement is complete.

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Q: Where is the thermostat located on a 2005 Toyota Highlander?
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