kaufman
There are many benefits associated with using a laser scanning confocal microscope. The main advantage is to obtain pictures one would not normally be able to receive at such depths.
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. cited works: wikipedia
it was discovered in the year 1595
The microscope was first discovered by Dutch spectacle makers Zacharias Janssen and his father Hans in the late 16th century. They developed an early version of the compound microscope using lenses in a tube.
Janne B. Arnaiz ^^
JFK
in the 1980's
There are many benefits associated with using a laser scanning confocal microscope. The main advantage is to obtain pictures one would not normally be able to receive at such depths.
Stereomicroscope, Compound Microscope, Phase-contrast microscope, electron microscope, Scanning-electron microscope, Transmission electron microscope, Confocal-scanning microscope. THESE ARE JUST SOME. :)
Compound ,Dissection or Stereoscope, Confocal Microscope, Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM).
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. cited works: wikipedia
There are at least two types of microscope that can give 3D images. Confocal microscopes that use lasers to illuminate the object and scanning electron microcopes (SEM) that use an electron beam. A SEM can give better magnification than confocal but confocal can image live moving subjects. In SEM the object of intrest must be coated with gold so only dead things can be imaged.
A confocal microscope would be best suited for observing the nucleus inside a living cell. Confocal microscopy uses laser beams to create high-resolution images with minimal damage to the specimen, making it ideal for studying structures within living cells. Additionally, confocal microscopes can generate three-dimensional images of the nucleus, providing detailed insights into its organization and function.
There are several type of microscopes, mainly, the one that we use in lab is a simple light microscope or a compound microscope. Then we have the phase contrast microscope, fluorescent microscope, electron microscope (transmission electron microscope [TEM] and scanning electron microscope [SEM]), confocal microscope and even dissection microscope the one which we use during dissections.
Confocal microscopes have scanning, and scan 1 point, where light has a whole feild of vision. Confocal laser scanning can get you a 3D image like tomography. you can scan a very thick sample and the microscope used is called a confocal microscope which uses a laser.
Robert Hooke in the year 1665 discovered the primitive microscope .
one Major difference is confocal microscopy has confocality which means it reduces the background signal which is not presented in conventional fluorescence microscope usually termed as epifluorescence microscope