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The duration a gallon of biofuel lasts depends on various factors, including the type of biofuel, the efficiency of the vehicle or machinery using it, and the distance traveled or energy consumed. For instance, a gallon of biodiesel might power a vehicle for about 20 to 40 miles, while a gallon of ethanol could provide similar mileage. Overall, the specific usage will greatly influence how long a gallon of biofuel will last.

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