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Using oil under 100x magnification enhances the resolution and clarity of the image by reducing light refraction and increasing the amount of light that enters the objective lens. This is particularly important for high-resolution microscopy, as it allows for better visualization of fine details in the specimen. Additionally, oil immersion helps to match the refractive index of the glass and the specimen, improving the overall quality of the microscopic image.
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What microscope objective is best to study bacteria?
A 100x oil immersion objective is best for studying bacteria under a microscope as it provides the highest magnification and resolution needed to observe bacterial morphology and structure in detail. Additionally, the use of oil immersion helps to reduce the refraction of light and improve image clarity.
When viewing a slide under oil immersion it is best to use which objective?
When viewing a slide under oil immersion, it is best to use the 100x objective lens. This high-power lens allows for greater magnification and resolution, making it ideal for observing fine details in the specimen. The use of immersion oil helps to reduce refraction of light and improves image clarity by providing a medium with a similar refractive index to glass.
A magnification of 100 requires the use of what objective?
A magnification of 100x typically requires the use of a 100x objective lens on a microscope. Different microscopes may have varying objective lens magnifications, but for a total magnification of 100x, the 100x objective is commonly used.
The total magnification achieved when using a 100x oil immersion lens with a 10x binocular eyepiece is?
The total magnification achieved when using a 100x oil immersion lens with a 10x binocular eyepiece is 1000x (100x objective magnification x 10x eyepiece magnification).
What micrscope objective is best to study bacteria?
100x with oil immersion
What objective focuses closest to slide?
The oil immersion lens @100x
What is the magnification of objects observed through a 100X oil immersion objective with a 7.5X eyepiece?
The total magnification would be 750X (100X objective multiplied by 7.5X eyepiece). Oil immersion objectives are specifically designed for use with immersion oil to minimize light refraction and increase resolution when viewing specimens with high magnification.
Can a specimen be viewed under a microscope using the 100x objective without a coverslip?
No, a specimen should not be viewed under a microscope using the 100x objective without a coverslip. The 100x objective requires a thin layer of immersion oil to properly focus light and achieve the necessary resolution. Without a coverslip, the specimen may be too far from the lens, resulting in poor image quality and potential damage to both the specimen and the objective lens.
Why is oil necessary when using the 100x objective?
Oil is necessary when using the 100x objective in a microscope to increase the resolution and clarity of the image. The oil has a similar refractive index to glass, reducing light refraction and increasing the numerical aperture, allowing for better resolution at high magnifications.
What is the magnification of a high power objective?
The oil immersion lens or objective has power 90X-100X and an eyepiece lens generally in light microscope comes with 10X so total magnification of oil immersion lens is 100X10 = 1,000
Why is 100x microscope lens called oil immersion lens?
Because that lens (it does not HAVE to be a 100x lens - other lens magnifications are also available in oil immersion constructions) is made to be in contact with a drop of special oil which covers the specimen. The oil has the same index of refraction as the front lens glass and that way a different optical construction can be used.
What is the function of the oil used with the oil immersion lens?
So that you do not scratch the lens because at 100x magnification it is very very close to the slide.
