No, it cannot. Defined media can only consist of pure chemicals added together. Since yeast extract is a digest of yeast, there is no way of knowing exactly what chemicals it contains. Substitutions in defined media typically consist of a carbon source (i.e. xylose) and any amino acids the sample requires.
A chemically defined media is a media in which the chemical nature of all the ingredients and their amounts are known. These media are also called synthetic media, mainly chemoorganotrophic heterotrophs also can be grown in defined media with glucose as a carbon source and an ammonium salt as a nitrogen source.
Complex media are preferable for routine cultivation of microorganisms than chemically defined media because it provides highly enriched sources such as plant or animal extracts, vitamins, minerals, protein, sugar, and or blood to the organism, supplying them specific nutritional substances needed for their growth. Chemically defined media on the other hand does not supply such a vast abundance of nutritional substances.
Sabouraud Agar is used as a selective media to isolate yeasts and molds. generally, use a complex kind of media, involving plant or animal extracts.
The nutreint media which reamin in liquid form through out the culturing process is called liquid media. It is different to solid media in not having agar in it which is the solidifying agent used in medium prepration. Commonly prepare liquid media is nutrient broth which contain beef extract, partially digest protein (peptones) and sodium chloride.
Complex culture media is a type of nutrient for growing bacteria that does not have exact composition. For example it may contain beef broth which has many nutritional components but the exact ratio of each component is not known. The opposite would be defined medium which contains exact amount of specific nutrients.
A chemically defined media is a media in which the chemical nature of all the ingredients and their amounts are known. These media are also called synthetic media, mainly chemoorganotrophic heterotrophs also can be grown in defined media with glucose as a carbon source and an ammonium salt as a nitrogen source.
In the complex media you have unknow compounds mixed in wheras; in the defined media you know all concentrations of the compounds in the media
Because complex media contain all nutrients, both known and unknown, which may be required for optimal growth of a wide variety of bacteria.
Chemically Composition is known & made with synthetic inorganic chemical substances.. Eg:- Glucose Broth, Inorganic Synthetic Broth
Complex media are preferable for routine cultivation of microorganisms than chemically defined media because it provides highly enriched sources such as plant or animal extracts, vitamins, minerals, protein, sugar, and or blood to the organism, supplying them specific nutritional substances needed for their growth. Chemically defined media on the other hand does not supply such a vast abundance of nutritional substances.
It's media made from scratch, with every single ingredient defined and listed separately. This media is used only when you want to determine which nutrients a microbe absolutely must have and which ones it can make. -The medium is chemically defined. This means that it only contains pure organic and pure inorganic compounds that are specified by an exact formula.The content varies little between sources. -Minimal media, for fungi, contain only a few essential compounds like salt and amino acids, other types of synthetic media are useful in research and cell culture when the exact nutritional needs of the test organisms are known.
-The medium is chemically defined. This means that it only contains pure organic and pure inorganic compounds that are specified by an exact formula.The content varies little between sources. -Minimal media, for fungi, contain only a few essential compounds like salt and amino acids, other types of synthetic media are useful in research and cell culture when the exact nutritional needs of the test organisms are known.
Sabouraud Agar is used as a selective media to isolate yeasts and molds. generally, use a complex kind of media, involving plant or animal extracts.
Yeast extract, Peptone, and Dextrose
why colony of agrobacterium is pink on yeast extract
Beef Extract Powder is prepared and standardized for use in microbiological culture media, where it is generally used to replace infusion of meat. Culture media containing Beef Extract Powder are recommended for use in bacteriological examination of water, milk, and other materials, where uniform composition of media is important. Beef Extract Powder is relied upon for biochemical studies, particularly fermentation reactions because of its independence from fermentable substances. Several media containing Beef Extract Powder are recommended in standard methods for multiple application.
Rena Frances Fruitman has written: 'A comparison of cell growth in chemically defined media, CMRL - 1415 and CMRL - 1066' -- subject(s): Cells, Tissue culture