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the bacteria dont ferment in MSA, there is no growth on the plat
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Why does gram-positive bacteria grow in mannitol agar?
Gram-positive bacteria grows in Mannitol agar because it contains a high level of salt. This type of agar allows only certain types of bacteria to grow, making it selective.Ê
What is the gram stain of enterobacter aerogenes?
Enterobacter aerogenes is a Gram negative rod-shaped bacterium in the same family as Esherichia coli. It can grow on many of the same selective media as Esherichia coli, including: MacConkey Agar, EMB agar and Lauryl-Tryptose broth. E. aerogenes ferments lactose, producing acid and gas like Esherichia coli and is classified as an example of coliform bacteria. There are several significant differrences between E. aerogenes and E. coli: 1) Most strains of E.coli are able to grow and produce acid from lactose in a medium such as lauryl tryptose broth or brilliant green bile broth at 44.5 degrees C. while most strains of E. aerogenes do not grow well at that temperature, E. aerogenes grows better at temperatures betwee 34 - 40 degrees C. 2) E. aerogenes carries out 2,3-butanediol fermentation and thus give a positive test in the Voges-Proskauer test while E. coli is negative. 3) E. coli is positive in the methyl red test while E. aerogenes is usually, but not always, negative (this is not the best test to rely on). 4) E.coli is positive for the indole test while E. aerogenes is negative, this is a very reliable test. 5) E. aerogenes can grow on Simmon's citrate agar while E. coli does not. You can distinguish between E. aerogenes and bacteria in the Proteus and Salmonella genera by using the hydrogen sulfide test, using either Kligler's iron agar or triple sugar iron agar. Most Proteus and Salmonella strains produce hydrogen sulfide and make a black precipitate but E. aerogenes does not. There are a lot of similarities between Enterobacter aerogenes and Klebsiella pneumonia. The urease test is one of the few tests that distinguishes E. aerogenes from K. pneumonia. Enterobacter aerogenes is a Gram negative rod-shaped bacterium in the same family as Esherichia coli. It can grow on many of the same selective media as Esherichia coli, including: MacConkey Agar, EMB agar and Lauryl-Tryptose broth. E. aerogenes ferments lactose, producing acid and gas like Esherichia coli and is classified as an example of coliform bacteria. There are several significant differrences between E. aerogenes and E. coli: 1) Most strains of E.coli are able to grow and produce acid from lactose in a medium such as lauryl tryptose broth or brilliant green bile broth at 44.5 degrees C. while most strains of E. aerogenes do not grow well at that temperature, E. aerogenes grows better at temperatures between 34 - 40 degrees C. 2) E. aerogenes carries out 2,3-butanediol fermentation and thus give a positive test in the Voges-Proskauer test while E. coli is negative. 3) E. coli is positive in the methyl red test while E. aerogenes is usually, but not always, negative (this is not the best test to rely on). 4) E.coli is positive for the indole test while E. aerogenes is negative, this is a very reliable test. 5) E. aerogenes can grow on Simmon's citrate agar while E. coli does not. You can distinguish between E. aerogenes and bacteria in the Proteus and Salmonella genera by using the hydrogen sulfide test, using either Kligler's iron agar or triple sugar iron agar. Most Proteus and Salmonella strains produce hydrogen sulfide and make a black precipitate but E. aerogenes does not. There are a lot of similarities between Enterobacter aerogenes and Klebsiella pneumonia. The urease test is one of the few tests that distinguishes E. aerogenes from K. pneumonia. Klebsiella is positive for urease production while Enterobacter is negative.
Can serratia marcescens grow in mannitol salt agar?
No it doesn't because it doesn't ferment mannitol. P. mirabilis can actually grow on a MAC agar, but it is colorless. I actually had this lab in Micro260 today.An MSA agar has 7% high salt concentration and provides growth for S. aureus bacteria turning it yellow/gold. An MSA agar is selective for osmotolerant bacteria meaning for bacteria that can grow in different salt concentrations, and it's differential because it can ferment mannitol.
Purpose of high salt concentration in the mannitol salt agar medium?
Mannitol salt agar (MSA) contains high levels of salt because it inhibits the growth of most bacteria. This makes it an excellent medium to test for Staphylococci and Micrococcaceae as they are tolerant of high levels of NaCl.
Which ingredient supplies carbon in the mannitol salt agar?
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
Can enterobacter aerogenes grow in TSA media?
yes
Can Klebsiella pneumoniae grow on Mannitol salt agar?
No, K. pneumoniae will not grow on Mannitol Salt Agar- gram negative organisms cannot grow on MSA, and K. pneumoniae is gram negative.
Why does gram-positive bacteria grow in mannitol agar?
Gram-positive bacteria grows in Mannitol agar because it contains a high level of salt. This type of agar allows only certain types of bacteria to grow, making it selective.Ê
What is the gram stain of enterobacter aerogenes?
Enterobacter aerogenes is a Gram negative rod-shaped bacterium in the same family as Esherichia coli. It can grow on many of the same selective media as Esherichia coli, including: MacConkey Agar, EMB agar and Lauryl-Tryptose broth. E. aerogenes ferments lactose, producing acid and gas like Esherichia coli and is classified as an example of coliform bacteria. There are several significant differrences between E. aerogenes and E. coli: 1) Most strains of E.coli are able to grow and produce acid from lactose in a medium such as lauryl tryptose broth or brilliant green bile broth at 44.5 degrees C. while most strains of E. aerogenes do not grow well at that temperature, E. aerogenes grows better at temperatures betwee 34 - 40 degrees C. 2) E. aerogenes carries out 2,3-butanediol fermentation and thus give a positive test in the Voges-Proskauer test while E. coli is negative. 3) E. coli is positive in the methyl red test while E. aerogenes is usually, but not always, negative (this is not the best test to rely on). 4) E.coli is positive for the indole test while E. aerogenes is negative, this is a very reliable test. 5) E. aerogenes can grow on Simmon's citrate agar while E. coli does not. You can distinguish between E. aerogenes and bacteria in the Proteus and Salmonella genera by using the hydrogen sulfide test, using either Kligler's iron agar or triple sugar iron agar. Most Proteus and Salmonella strains produce hydrogen sulfide and make a black precipitate but E. aerogenes does not. There are a lot of similarities between Enterobacter aerogenes and Klebsiella pneumonia. The urease test is one of the few tests that distinguishes E. aerogenes from K. pneumonia. Enterobacter aerogenes is a Gram negative rod-shaped bacterium in the same family as Esherichia coli. It can grow on many of the same selective media as Esherichia coli, including: MacConkey Agar, EMB agar and Lauryl-Tryptose broth. E. aerogenes ferments lactose, producing acid and gas like Esherichia coli and is classified as an example of coliform bacteria. There are several significant differrences between E. aerogenes and E. coli: 1) Most strains of E.coli are able to grow and produce acid from lactose in a medium such as lauryl tryptose broth or brilliant green bile broth at 44.5 degrees C. while most strains of E. aerogenes do not grow well at that temperature, E. aerogenes grows better at temperatures between 34 - 40 degrees C. 2) E. aerogenes carries out 2,3-butanediol fermentation and thus give a positive test in the Voges-Proskauer test while E. coli is negative. 3) E. coli is positive in the methyl red test while E. aerogenes is usually, but not always, negative (this is not the best test to rely on). 4) E.coli is positive for the indole test while E. aerogenes is negative, this is a very reliable test. 5) E. aerogenes can grow on Simmon's citrate agar while E. coli does not. You can distinguish between E. aerogenes and bacteria in the Proteus and Salmonella genera by using the hydrogen sulfide test, using either Kligler's iron agar or triple sugar iron agar. Most Proteus and Salmonella strains produce hydrogen sulfide and make a black precipitate but E. aerogenes does not. There are a lot of similarities between Enterobacter aerogenes and Klebsiella pneumonia. The urease test is one of the few tests that distinguishes E. aerogenes from K. pneumonia. Klebsiella is positive for urease production while Enterobacter is negative.
Can serratia marcescens grow in mannitol salt agar?
No it doesn't because it doesn't ferment mannitol. P. mirabilis can actually grow on a MAC agar, but it is colorless. I actually had this lab in Micro260 today.An MSA agar has 7% high salt concentration and provides growth for S. aureus bacteria turning it yellow/gold. An MSA agar is selective for osmotolerant bacteria meaning for bacteria that can grow in different salt concentrations, and it's differential because it can ferment mannitol.
Can Streptococcus grow on MSA?
MSA stands for mannitol salt agar. Streptococcus can not grow on this type of agar. This is because it has a very high salt content, which allows only certain microorganisms to grow in it.
How do mannitol-salt agar tests work?
Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.
What selective agent found in Mannitol Salt Agar?
The high salt concentration (7.5-10% w/v) makes the agar hard for all but staph spp. To grow, and staph aureus turns yellow when it ferments the mannitol.
Did lactobacillus grow on mannitol salt agar?
Yes. M. smegmatis is a gram + bacteria, and MSA plates select for gram + bacteria.
Purpose of high salt concentration in the mannitol salt agar medium?
Mannitol salt agar (MSA) contains high levels of salt because it inhibits the growth of most bacteria. This makes it an excellent medium to test for Staphylococci and Micrococcaceae as they are tolerant of high levels of NaCl.
What are the example of selective media?
Examples of selective media include MacConkey agar, Eosin methylene blue agar, and Mannitol salt agar. These types of media contain ingredients that selectively inhibit the growth of certain organisms while allowing the growth of others.
Which ingredient supplies carbon in the mannitol salt agar?
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
