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No bacteria exist without a cell wall! If they loose cell wall then they die. Gram staining is used to differentiate gram positive (that doesnt have outer membrane) and gram negative (cell wall+outer membrane) bacteria.

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Which part of bacteria cell is most involved in gram stain?

The cell wall of a bacterial cell is the most involved in the Gram stain because it determines whether the bacteria will appear purple (Gram-positive) or pink (Gram-negative) after staining. The composition of the cell wall, specifically the presence of peptidoglycan, influences how the stain interacts with the bacteria.


What organism doesn't stain on a gram stain?

Mycoplasma is an example of an organism that does not stain on a Gram stain due to its lack of a cell wall. Mycoplasma is classified as a bacterium, but its unique cell wall composition causes it to not retain the crystal violet stain in the Gram staining process.


What are the differences between the Gram stain and acid-fast stain techniques in microbiology?

The Gram stain technique is used to differentiate bacteria into two groups based on their cell wall composition (Gram-positive and Gram-negative), while the acid-fast stain technique is used to detect bacteria that have a waxy cell wall, such as Mycobacterium species.


Bacteria are divided into two groups based on how their cell walls absorb?

Bacteria are divided into two groups based on how their cell walls absorb the Gram stain. Gram-positive bacteria retain the stain due to their thick peptidoglycan cell wall, while Gram-negative bacteria do not retain the stain because of their thin peptidoglycan cell wall and an outer membrane.


What causes a stain to adhere to bacterial cells?

Stains adhere to bacterial cells due to the physical properties of both the stain and the cell wall components. For example, crystal violet in the Gram stain adheres to the peptidoglycan layer in Gram-positive bacteria due to electrostatic interactions. Once the stain binds to the cell wall, it is retained during the washing steps in the staining process.

Related Questions

What is the difference between gram-positive and gram-negative bacteria?

The presence of thick cell wall. Gram positives have a thick cell wall and remain blue. Gram negatives have a thin cell wall where the blue stain washes out and then will stain pink with the counter stain.


Which part of bacteria cell is most involved in gram stain?

The cell wall of a bacterial cell is the most involved in the Gram stain because it determines whether the bacteria will appear purple (Gram-positive) or pink (Gram-negative) after staining. The composition of the cell wall, specifically the presence of peptidoglycan, influences how the stain interacts with the bacteria.


What organism doesn't stain on a gram stain?

Mycoplasma is an example of an organism that does not stain on a Gram stain due to its lack of a cell wall. Mycoplasma is classified as a bacterium, but its unique cell wall composition causes it to not retain the crystal violet stain in the Gram staining process.


Are polymers gram negative?

A gram stain tests the contents of the cell wall. Polymers do not have cell walls.


What is the Gram stain for measles?

Bacteria stain either gram-positive or gram-negative based on the presence or absence of a cell wall. Viruses do not pick up a gram stain.


What are the differences between the Gram stain and acid-fast stain techniques in microbiology?

The Gram stain technique is used to differentiate bacteria into two groups based on their cell wall composition (Gram-positive and Gram-negative), while the acid-fast stain technique is used to detect bacteria that have a waxy cell wall, such as Mycobacterium species.


Which thing make gram positive bacteria to get stain?

the smooth part of the cell wall make it to get stain


Bacteria are divided into two groups based on how their cell walls absorb?

Bacteria are divided into two groups based on how their cell walls absorb the Gram stain. Gram-positive bacteria retain the stain due to their thick peptidoglycan cell wall, while Gram-negative bacteria do not retain the stain because of their thin peptidoglycan cell wall and an outer membrane.


What causes a stain to adhere to bacterial cells?

Stains adhere to bacterial cells due to the physical properties of both the stain and the cell wall components. For example, crystal violet in the Gram stain adheres to the peptidoglycan layer in Gram-positive bacteria due to electrostatic interactions. Once the stain binds to the cell wall, it is retained during the washing steps in the staining process.


Did you stain the bacterial cell wall or the cell cytoplasm?

Yes, it is. Using the Gram staining method, the wall can be seen.


What is the gram stain for measles virus?

Measles virus is a negative-sense, single-stranded RNA virus and does not have a cell wall structure that can be stained with the Gram stain. Instead, measles virus particles can be visualized using specialized staining techniques such as immunofluorescence or electron microscopy.


Does the gram stain procedure work for all bacteria?

Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.