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Pulse field gel electrophoresis is used to separate DNA fragments by their size.
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∙ 10y ago
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∙ 12y agoElectrophoresis.... im 100% sure because I just turned into my online class and got it right
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∙ 12y agoGel electrophoresis, like SDS PAGE can be used.
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∙ 12y agogel electrophoresis
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∙ 12y agoRestrictive Enzymes
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Difference between southern and northern blotting?
Southern Blotting refers to the identification of detailed sequences of DNA in which the DNA fragments are separated by electrophoresisNorthern Blotting refers to the identification of detailed sequences of RNA in which the RNA fragments are separated by electrophoresis
Properties of DNA fragments which allow them to be separated from each other during gel electrophoresis?
They are negatively charged and are of different sizes
What enzyme binds fragments of DNA on the lagging strand?
The enzyme that is used to bind DNA fragments together is DNA ligase. Using DNA ligase to join DNA fragments is the last step in the production of a recombinant DNA plasmid.
How are samples separated on a gel?
Fragments are separated by size with smaller fragments migrating more quickly than larger ones.
What is maxam Gilbert method of DNA packaging?
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
How are DNA fragments separated on DNA fingerprinting?
Gel electrophoresis
Difference between southern and northern blotting?
Southern Blotting refers to the identification of detailed sequences of DNA in which the DNA fragments are separated by electrophoresisNorthern Blotting refers to the identification of detailed sequences of RNA in which the RNA fragments are separated by electrophoresis
What is the pattern of dark bands on photographic film that is made when DNA fragments are separated by gel electrophoresis and tagged?
DNA fingerprint
Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
Are the DNA fragments in band 4 larger or smaller than those of band 1?
The size of DNA fragments in band 4 should be smaller than those of band 1. The fragments can be separated by electrophoresis, with the smaller fragments migrating farther than the larger ones.
Why are D1S80 fragments separated by gel electophoresis?
Fragments are separated by gel electrophoresis because of their differing sizes. DNA is negatively charged, so will migrate through the gel towards the positive electrode. The smaller fragments are able to move through the gel more quickly than the larger fragments - which means they separate based on their size.
Properties of DNA fragments which allow them to be separated from each other during gel electrophoresis?
They are negatively charged and are of different sizes
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
How do you get DNA fragments in Bakugan dimensions?
You get DNA fragments by entering Bakugan codes.
What do you do with DNA fragments in bakugan dimensions?
When You collect 20 DNA fragments you get a free bakugan
What are DNA fragments that have attached RNA fragments called?
Okazaki fragments.
