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First you have to understand that the purpose of this technique is to observe living bacteria being motile. Motility is an inheritable phenotype and is critical for identification and classification of bacteria. The technique is done by applyling petroleum jelly to all sides of a cover glass. Add a water droplet and specimen using an aseptic loop onto the concave side of a depression slide. Put the jelly side of the cover glass over the droplet. View the droplet as it is suspended or "hanging" from the side. It is a "hanging drop" slide because the droplet remains untouched due to the concave shape of the cover glass and it just hangs from the cover glass. First you have to understand that the purpose of this technique is to observe living bacteria being motile. Motility is an inheritable phenotype and is critical for identification and classification of bacteria. The technique is done by applyling petroleum jelly to all sides of a cover glass. Add a water droplet and specimen using an aseptic loop onto the concave side of a depression slide. Put the jelly side of the cover glass over the droplet. View the droplet as it is suspended or "hanging" from the side. It is a "hanging drop" slide because the droplet remains untouched due to the concave shape of the cover glass and it just hangs from the cover glass.
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Does the hanging-drop technique have any practical value?
hanging drop tecnique have practical value
What is the value of a hanging-drop preparation?
Hanging drop preparation is very valuable as it allows for a truer research capability. Hanging drop preparation offers longer observation abilities.
Purpose of the hanging drop.?
A hanging-drop is used to determine bacterial motility, besides brownian movement.
What specimen preparation is best for viewing cell motility?
hanging drop
Hanging drop slide?
To make a hanging drop slide, you will need: -a depression slide -a square coverslip -some petrolatum -the liquid suspension of what you wish to view Place a small spot of petrolatum on each of the 4 corners of the coverslip. Place a drop of your suspension in the center of the coverslip. Invert a depression slide over the drop, allowing the petrolatum to attach the coverslip to the depression slide. Quickly (but carefully) invert the slide so that the coverslip is oriented "up", and the drop is hanging into the slide depression.
Does the hanging-drop technique have any practical value?
hanging drop tecnique have practical value
Does hanging drop technique have any practical value?
hanging drop tecnique have practical value
What is the purpose of hanging drop technique?
The purpose of the hanging drop technique is a method in which a drop of bacterial suspension is enclosed in an air-tight chamber prepared in a special depression slide. It allows you to view living organisms.
What is the value of a hanging-drop preparation?
Hanging drop preparation is very valuable as it allows for a truer research capability. Hanging drop preparation offers longer observation abilities.
Purpose of the hanging drop.?
A hanging-drop is used to determine bacterial motility, besides brownian movement.
What is the use of hanging drop?
Hanging drop preparations can be observed for a long time because they do not dry up very quickly. Thus, motility of an organisim can be observed.
Aside from motility what facts learned from hanging drop preparation?
The hanging drop preparation can study the motility over a longer period of time, but it is more difficult to examine than a wet mount. Wet mount is preferable to a hanging drop slide when using phase contrast optics.
Why isn't the oil immersion lens used in hanging drop procedure?
the hanging drop procedure uses a thicker slide, which could crack if the oil immersion lens is used. My bio professor worte: hanging drop slides asre too thick to use under oil.
What is the definition for paper electrophoresis?
it is a separation technique
What is the silver line in hanging drop method?
The sliver line that you see is actually an artifact and the edge of the drop.
How does hanging cause?
Hanging as a form of capital punishment is achieved by the drop and the neck is broken. Lynching some one chokes them to death.
Would you expect Brownian motion to increase the longer you observe a hanging drop preparation?
Just by the sheer fact that any vibration of any kind can be considered Brownian motion for a hanging drop test. the longer you observe your bacteria, the more chance there are of Brownian motion interfering with your motility test ( I assume you're talking about the motility hanging drop, right?). It is because of this fact that the hanging drop test is quickly falling out of favor for more secure tests for motility, the semi-solid media being one of them.
