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Two populations are isolated geographically (or mechanically - perhaps there is a mutation that prevents physical copulation, like a change in the direction of the turn of snail shells). One undergoes some selective pressure that the other does not, such as a change in the environment or new predators moving in. The population under stress adapts to the changes, and the genetic mutations involved are extensive enough such that if the two populations do meet again, they can no longer produce viable offspring.

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