There are many disadvantages to stain and not staining cells before viewing them. One disadvantage of gram staining is that the cells cannot be alive while being stained, so viewing live stained cells is impossible.
The disadvantages of staining the cells is that the cells gets killed. This therefore means that some functions like the streaming of protoplasm cannot be studied.
the stain kills the cell, making it impossible to view a living cell
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Chromatin coils and condenses to form deeply-staining bodies in prophase. This is the first stage of mitosis, the process in which cells divide to create new cells.
One of the disadvantages of heating fixing is that it is very expensive. Secondly, it cannot be used in capsular staining.
Most cells contain structures that are transparent and without color, which makes visualization difficult, even with the aid of a microscope. Cell staining is used to gain more information about cells and tissues, and allows for the structures within cells to be seen in great detail
If your talking about Acid Fast staining (aka Ziehl-Neelsen staining), after the addition of the primary stain (carbol fuchsin) heat is applied in order to facilitate proper staining. Bacteria such as Mycobacterium contain large amounts of lipid substances within their cell wall called mycolic acids. These acids resist staining by ordinary methods such as gram staining (where heat is not applied after primary staining). On application of heat, the stain carbol fuschin penetrates the cell wall and stains the cells pink. Following the secondary staining (methylene blue) the acid fast positive cells appear pink while others are stained blue. Endospore staining is yet another staining technique where heat is applied after primary staining (malachite green). In this case the spores are impermeable to stains, hence heating favours proper permeation of stain. Endospores appear green while vegetative cells appear red (secondary stain saffranine). Not all staining procedures involve applying heat after primary staining. However, heat is applied before even beginning the staining procedure. This is called heat fixing, where the cells are fixed so that they are not washed away during the subsequent washing steps.
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Advantages: easy to carry out, natural shape and side Disadvatages: heat fixing can distorte cells, more attention needed
what are the advantages and disadvantages primary cells?
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they are easier to see when you use a microscope
to kill cells
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
The combination of Congo red cream and distilled water is used for staining cells for viewing under the microscope. Staining make the individual cells much easier to see.
Chromatin coils and condenses to form deeply-staining bodies in prophase. This is the first stage of mitosis, the process in which cells divide to create new cells.
There are several uses for a staining jar. In microscopy, it is used for staining tissues and cells for slides. After being stained with dyes or stains, the specimens can also be placed in the jar to look for certain aspects.
It sometimes require additional chemical reagents to produce the desired action.
It will make them stand out more under a microscope