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Disadvantages of staining cells

Updated: 8/9/2023
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9y ago

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There are many disadvantages to stain and not staining cells before viewing them. One disadvantage of gram staining is that the cells cannot be alive while being stained, so viewing live stained cells is impossible.

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9y ago
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9y ago

The disadvantages of staining the cells is that the cells gets killed. This therefore means that some functions like the streaming of protoplasm cannot be studied.

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12y ago

the stain kills the cell, making it impossible to view a living cell

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Is endospore staining differencial staining?

Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.


Chromatin coils and condenses to form deeply staining bodies?

Chromatin coils and condenses to form deeply-staining bodies in prophase. This is the first stage of mitosis, the process in which cells divide to create new cells.


What are the disadvantages of heat fixing?

One of the disadvantages of heating fixing is that it is very expensive. Secondly, it cannot be used in capsular staining.


What is the importance of staining specimen for observation under the microscope?

Most cells contain structures that are transparent and without color, which makes visualization difficult, even with the aid of a microscope. Cell staining is used to gain more information about cells and tissues, and allows for the structures within cells to be seen in great detail


What is a primary stain?

If your talking about Acid Fast staining (aka Ziehl-Neelsen staining), after the addition of the primary stain (carbol fuchsin) heat is applied in order to facilitate proper staining. Bacteria such as Mycobacterium contain large amounts of lipid substances within their cell wall called mycolic acids. These acids resist staining by ordinary methods such as gram staining (where heat is not applied after primary staining). On application of heat, the stain carbol fuschin penetrates the cell wall and stains the cells pink. Following the secondary staining (methylene blue) the acid fast positive cells appear pink while others are stained blue. Endospore staining is yet another staining technique where heat is applied after primary staining (malachite green). In this case the spores are impermeable to stains, hence heating favours proper permeation of stain. Endospores appear green while vegetative cells appear red (secondary stain saffranine). Not all staining procedures involve applying heat after primary staining. However, heat is applied before even beginning the staining procedure. This is called heat fixing, where the cells are fixed so that they are not washed away during the subsequent washing steps.

Related questions

Is endospore staining differencial staining?

Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.


What are the advantages and disadvantages of positive ands negative staining for morphological study of bacteria?

Advantages: easy to carry out, natural shape and side Disadvatages: heat fixing can distorte cells, more attention needed


Advantages and disadvantages of primary cells?

what are the advantages and disadvantages primary cells?


What careers take part in staining cells?

Tattoo artist. (BTP)


What advantage can be gained from staining cells?

they are easier to see when you use a microscope


Why is it imortant to immoblize the bacteria on the slide prior to staining?

to kill cells


What is thee purpose of adding methylene blue to the specimen?

Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.


Congo Red Cream and Distilled Water?

The combination of Congo red cream and distilled water is used for staining cells for viewing under the microscope. Staining make the individual cells much easier to see.


Chromatin coils and condenses to form deeply staining bodies?

Chromatin coils and condenses to form deeply-staining bodies in prophase. This is the first stage of mitosis, the process in which cells divide to create new cells.


What is the function of staining jar?

There are several uses for a staining jar. In microscopy, it is used for staining tissues and cells for slides. After being stained with dyes or stains, the specimens can also be placed in the jar to look for certain aspects.


What are the disadvantages of negative staining in microbiology?

It sometimes require additional chemical reagents to produce the desired action.


What effect does staining have on plant cells?

It will make them stand out more under a microscope