No, Eukayotic transcription begins after promoter clearance. Promoter clearance just prepares the transcription initiation complex to begin elongation. Promoter clearance does not produce any functional gene.
The TATA box seems to help position RNA polymerase by marking a point just before the point at which transcription begins.
Sections of RNA molecules that are removed before a eukaryotic gene becomes functional introns. The signal in DNA that indicates to enzymes where to bind to make RNA is the promoter.
Bacteria can begin translation before transcription has terminated.
It must unwind and separate for transcription to occur
DNA strands must unwind and the two strands must separate prior to transcription beginning. Once transcription is complete, the two strands join back together.
The TATA box seems to help position RNA polymerase by marking a point just before the point at which transcription begins.
Sections of RNA molecules that are removed before a eukaryotic gene becomes functional introns. The signal in DNA that indicates to enzymes where to bind to make RNA is the promoter.
Bacteria can begin translation before transcription has terminated.
Gene regulation can occur in eukaryotic cells before, during, and after transcription.
during translation
It must unwind and separate for transcription to occur
DNA strands must unwind and the two strands must separate prior to transcription beginning. Once transcription is complete, the two strands join back together.
Clearance Delivery is contacted to receive IFR clearance or VFR departure instructions (departures clearance) . Pilots would do this after listening to the ATIS and before contacting ground for their taxi clearance.
Prokaryotes have a single circular chromosome, wherease eukaryotes have many bar shaped chromsomes. Also, prokaryotes are single cell organisms (there are some single cell eukaryotes, but never multicellular prokaryotes). Because multicellular organisms have so many cells, (such as humans), their chromsomes have to contain a lot of information to provide details to the cell so it can carry out it's job. In eukaryotic cell DNA there are introns and exons. When a gene is needed to be expressed, the cell must remove the information in the sequence that is not needed; ie, the introns. It is believed that the same DNA sequence can actually code for more than one gene because of the cells ability to remove introns and keep exons. for example, the word 'strawberry' contains the code for three words: straw, berry, strawberry. by removing peices of the word, you are left with information that provides a different set of instructions. Prokaryotes do not have introns and exons.
dna
Yes. Both eukaryotes and prokaryotes use the same system of codons (they speak the same 'language'). For example, GGG will be translated to glycine in a human and in E. coli bacteria. Therefore, as long as the eukaryotic gene is attached to the appropriate prokaryotic gene regulation elements, a prokaryotic cell will be able to translate and express the gene. The most common method to accomplish this is to put the eukaryotic gene in a 'vector' (most commonly a plasmid of bacterial origin), and then transform the bacteria with this vector.This is how humulin (human insulin) was first prepared by recombinant DNA technology. The eukaryotic genetic information (human DNA) was inserted into prokaryotic plasmids, and the bacteria then synthesized insulin.
No, Transcription does not require Replication to take place first. Transcription is simply the process of making mRNA from DNA so that the ribosomes have directions to make proteins. Replication is the complete copy of the genetic material in the host chromosome which would occur before the cell divides.