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It is not exactly an enzyme that makes poison ivy poisonous, rather a mix of chemicals known as Urushiol. Urushiol is an organic oil toxin found in plants of the Family Anacardiaceae (e.g. poison oak, poison ivy, and poison sumac). It causes the allergic skin rash on contact, known as urushiol-induced contact dermatitis.

Urushiol is a mixture of organic molecules appearing together as a yellow liquid. Each consists of a catechol substituted with an alkyl chain that has 15 or 17 carbon atoms. The alkyl group may be saturated or unsaturated; urushiol oil is a mixture of saturated and unsaturated molecules. The mixture varies with the vegetative species. Whereas Western poison oak urushiol contains chiefly catechols with C17 side chains, poison ivy and poison sumac yield mostly catechols with C15 side chains. The allergic reaction is dependent on the degree of unsaturation of the alkyl chain. Less than half of the general population reacts with the saturated urushiol alone, but over 90% react with urushiol containing at least two degrees of unsaturation (double bonds).

The toxic effects of urushiol are indirect, mediated by an induced autoimmune response. Urushiol chemically reacts with, binds to and changes the shape of integral membrane proteins on exposed skin cells. Affected proteins interfere with the immune system's ability to recognize these cells as normal parts of the body (perhaps causing the immune system to mistake them for invading parasites), causing a T-cell mediated immune response. This immune response is directed towards the complex of urushiol derivatives bound in the skin proteins, attacking the cells as if they were foreign bodies.

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