For a scanning electron microscope, the most commonly used type of EM, there is no need to do anything other than stick it to an electrically conducting pad. If the sample is particularly insulating, it may be worth coating it with a very thin gold layer to prevent the specimen from charging up with electrons from the beam (this would blur the image)
For a transmission electron microscope, the specimen is most commonly encased in epoxy resin before being sliced into thin enough slices that the electron beam can pass through. The sample is then placed on a fine copper grid before being placed in the microscope.
what did you consider in preparing spicimen for microscopic study?
they are soaked in an acidic solution and then left for 1 hour at the most.
Sliced and stained (dead)
"In electron microscope the object is viewed in highly vaccum conditions. Living cells would disentegrate in such a condition." This answer is not mine it comes from the "why can't electron microscopes be used to view cell structures" question
Yes, you can.
In a pound of a salty water
Take a clean slide (either depressed or flat) and add a drop of water using an eye dropper. Add the specimen and using the slip cover, touch the end of the slip cover to the water. Lay flat as smoothly as possible to omit bubbles from forming.
Because an air bubble, trapped between the slide and cover plate, shows as a dark'blob' under magnification. Using a drop of water to 'seal' the slide & cover plate together excludes the air - making the specimen more visible to the user.
They must be preserved and dehydrated. Once this is accomplished, put the specimen onto a slide, and then the slide is ready to be viewed under the microscope.
There there are many. Simple microscope, compound microscope, light microscope, scanning electron microscope, TEMicroscope, Dissection microscope, etc and most of them are used to see small cells that cannot be seen by the naked eye.
"In electron microscope the object is viewed in highly vaccum conditions. Living cells would disentegrate in such a condition." This answer is not mine it comes from the "why can't electron microscopes be used to view cell structures" question
Yes, you can.
spit on it
Gram-positive and gram-negative refer to the Gram stain used to prepare slides of bacteria for viewing under a light microscope. Viruses are too small to see under a light microscope and have to be prepared differently for viewing under an electron microscope. So the answer to your question is neither
Glycerine is quite isotonic to the cell. So, it does not damage the specimen.
Wrap the electrical cord around the arm of the microscope.
In a pound of a salty water
explain the key issues relating to the practice which supports children to prepare for transititons
Because an air bubble, trapped between the slide and cover plate, shows as a dark'blob' under magnification. Using a drop of water to 'seal' the slide & cover plate together excludes the air - making the specimen more visible to the user.
Take a clean slide (either depressed or flat) and add a drop of water using an eye dropper. Add the specimen and using the slip cover, touch the end of the slip cover to the water. Lay flat as smoothly as possible to omit bubbles from forming.