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The smallest organism is probably one of the retroviruses, which stand on the border between Things That Are Alive and Things That Are Just Chemical. The largest might be a stand of about 25,000 aspen trees with are all genetically identical and whose roots are interconnected, suggesting that in a sense, they're all the same tree.

Another answer:

Viruses are not alive no matter how small nor are they organisms. So the smallest organism is a bacteria called Candidatus tremblaya which is a betaproteobacteria. It is related to the bacteria that causes gonorrhea.

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