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What kind of reaction is DNA replication and what does it require?
DNA replication is a semi-conservative process where a DNA molecule makes a copy of itself. It requires enzymes such as DNA polymerase, dNTPs (deoxynucleotide triphosphates), a template DNA strand, and primer to initiate the process.
What role do ddNTPs play in the process of DNA replication?
During DNA replication, ddNTPs (dideoxynucleotide triphosphates) are used to terminate the growth of DNA strands by preventing the addition of more nucleotides. This is important in techniques like Sanger sequencing, where ddNTPs are used to create DNA fragments of different lengths for analysis.
How do single strand binding proteins contribute to the process of DNA replication?
Single strand binding proteins stabilize the unwound DNA strands during replication by preventing them from re-forming into a double helix. This allows other enzymes and proteins involved in replication to access the single-stranded DNA and carry out the replication process efficiently.
Why is the replication process is a source of few mutations?
Some mutations are due to errors in DNA replication. During the replication process, DNA polymerase chooses complementary nucleotide triphosphates from the cellular pool. Then the nucleotide triphosphate is converted to a nucleotide monophosphate and aligned with the template nucleotide. A mismatched nucleotide slips through this selection process only onece per 100,000 base pairs at most. The mismatched nucleotide causes a pause in replication, during which it is excised from the daughter strand and replaced with the correct nucleotide. After this so-called proofreading has occurred, the error rate is only one per 1 billion base pairs.
How does base pairing contribute to the process of DNA replication?
Base pairing contributes to the process of DNA replication by ensuring that each new strand of DNA is complementary to the original strand. This allows for accurate copying of genetic information during cell division.
Why are ribonucleotide triphosphates rather than ribonucleotides the monomers required for RNA synthesis in cells?
Ribonucleotide triphosphates are used as monomers for RNA synthesis because they provide energy for polymerization reactions and help drive the synthesis process forward. The incorporation of a ribonucleotide triphosphate into the growing RNA strand releases pyrophosphate, which helps drive the reaction towards completion. Additionally, ribonucleotide triphosphates are readily available in the cell due to their involvement in various cellular processes, making them suitable building blocks for RNA synthesis.
What kind of reaction is DNA replication and what does it require?
DNA replication is a semi-conservative process where a DNA molecule makes a copy of itself. It requires enzymes such as DNA polymerase, dNTPs (deoxynucleotide triphosphates), a template DNA strand, and primer to initiate the process.
What role do ddNTPs play in the process of DNA replication?
During DNA replication, ddNTPs (dideoxynucleotide triphosphates) are used to terminate the growth of DNA strands by preventing the addition of more nucleotides. This is important in techniques like Sanger sequencing, where ddNTPs are used to create DNA fragments of different lengths for analysis.
How do single strand binding proteins contribute to the process of DNA replication?
Single strand binding proteins stabilize the unwound DNA strands during replication by preventing them from re-forming into a double helix. This allows other enzymes and proteins involved in replication to access the single-stranded DNA and carry out the replication process efficiently.
Why is the replication process is a source of few mutations?
Some mutations are due to errors in DNA replication. During the replication process, DNA polymerase chooses complementary nucleotide triphosphates from the cellular pool. Then the nucleotide triphosphate is converted to a nucleotide monophosphate and aligned with the template nucleotide. A mismatched nucleotide slips through this selection process only onece per 100,000 base pairs at most. The mismatched nucleotide causes a pause in replication, during which it is excised from the daughter strand and replaced with the correct nucleotide. After this so-called proofreading has occurred, the error rate is only one per 1 billion base pairs.
How does base pairing contribute to the process of DNA replication?
Base pairing contributes to the process of DNA replication by ensuring that each new strand of DNA is complementary to the original strand. This allows for accurate copying of genetic information during cell division.
How does RNA polymerase contribute to the process of DNA replication?
RNA polymerase is not directly involved in DNA replication. Instead, it is responsible for transcribing DNA into RNA during the process of gene expression. DNA replication is carried out by a different enzyme called DNA polymerase, which synthesizes new DNA strands using the existing DNA as a template.
Replication in a sentence?
Our replication process has lost it's momentum.
What specific term describes the process when Dna replicates itself?
The specific term for the process when DNA replicates itself is called DNA replication. This process involves the complementary base pairing of nucleotides to create two identical copies of the original DNA molecule.
In prokaryotes where does DNA replication occur in the cell?
In prokaryotes, DNA replication occurs in the cytoplasm. The replication process begins at the origin of replication on the DNA molecule and proceeds bidirectionally. Multiple replication fork structures are formed to speed up the replication process.
Does replication require oxygen?
No, replication does not require oxygen. Replication is the process of copying DNA or RNA, which can occur in the absence of oxygen. Oxygen is not directly involved in the process of DNA replication itself.
How do binding proteins contribute to the process of DNA replication?
Binding proteins play a crucial role in DNA replication by attaching to specific sites on the DNA strand and helping to stabilize the replication process. They help to unwind the double helix structure of the DNA, allowing other enzymes and proteins to access the DNA strand and replicate it accurately. Binding proteins also prevent the DNA strands from rejoining prematurely, ensuring that the replication process proceeds smoothly and without errors.
