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Blue-white screening is a technique used in genetic engineering to identify successful DNA insertions into a host cell. The host cell contains a gene that produces an enzyme called -galactosidase, which turns a substrate called X-gal blue. When a DNA insert is successfully integrated into the host cell, it disrupts the -galactosidase gene, preventing the cell from turning X-gal blue. This results in white colonies, making it easy to identify cells that have taken up the desired DNA.

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6mo ago

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