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A plant cell can be isolated by using different priscribed techniques keeping in mind the sourse of isolation. For eg. to isolate a leaf cell first:- collect the leaf, homogenase it (crush it) in proper medium then add the mixture in centrifugation tube and centrifuge it repeatedly for 2 to 3 times. discard the supernetant and inoculate the debris in suitable culture media keeping the auxin and cytokinin ratio equal. CALLUS formation take place which is mass of undifferentiated cells. Now from this callus we can isolate plant cells.

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16y ago

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