How do you locate genes on the chromosomes of humans?

Answer 1

Traditionally, radiolabeled oligonucleotides were used to locate genes on chromosomes. In this method, oligonucleotide sequences that are complimentary to a section of the gene being located are labelled with a radioactive isotope - P32. Following annealing, the gene location is detected through auto-radiography.

These days, fluorescent labels are used. These labels are more versatile and easy to detect. They also overcome the risks of using radioactivity.

Answer 2

Topcat Says: Fluorescent in situ hybridization is a technique used to identify the location of a gene or a specific DNA sequence on a chromosome. The technique involves hybridizing single-stranded DNA probes, labeled with a fluorescent dye, to their complementary DNA strand in the genome. Their location on the chromosome can be viewed under a fluorescent microscope.

Answer 3

Genes on human chromosomes can be located using techniques such as DNA sequencing and genetic mapping. By analyzing the DNA sequence of a specific region of a chromosome and comparing it to known gene sequences, researchers can identify and locate genes. Genetic maps of chromosomes provide information on the relative positions of genes based on recombination frequency.

Answer 4

I'm not sure how the mapping process is done, but in order to find a locus on a chromosome map, geneticists analyze the recombination data.