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How does a freezing microtome work?

Updated: 4/28/2022
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Since most biological specimens are too squishy to cut at room temperature, a freezing microtome, also known as a cryostat, freezes the specimen to harden it so it can be sliced without ripping and destroying it.

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Q: How does a freezing microtome work?
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What is a freezing microtome?

A freezing microtome is an instrument used in the laboratory to section small delicate materials. Sectioning is done on a platform which is frozen by snowing CO2. The microtome knife is also snowed with CO2. In this type of microtome, sectioning is done by the microtome knife moving towards the material to be sectioned. For materials that have alcohol, it should first be removed before being sectioned.


What are different types of microtomes?

1. Sledge microtome. 2. Rocking microtome. 3. Rotary microtome. 4. Sliding microtome. 5. Ultra microtome.


'uses of microtome in histology'?

Microtomes are used to cut very thin slices of a specimen in order to examine the specimen microscopically.


What is a hand microtome?

Dear Friends, The word "microtome" is coined from the ancient Greek; "Micro" means "small" and "Tome" is a root from the verb "to cut." So a microtome is a device for making small cuts, actually thin slices, of material we wish to observe in the microscope. (Small and see). Most of the time, a microscope specimen has to be so thin that light will shine through it. So most plant and animal tissues have to be cut really thin. You need a finely sharpened knife, and the plant and animal tissue has to have a crisp, firm, but not too hard texture. If you know how to whittle with a jack-knife, you can cut many plant and animal tissues by hand and eye. It is not really that hard or dangerous. The hand microtome is a hollow tube with a screw adjustment on one end and a flat cutting surface on the other. You stick your specimen in the hole, advance the screw, and slide your knife across the specimen using the cutting surface as a guide. It is a very handy little instrument to help make your sections evenly thin. Advanced professionals, like pathologists use highly advanced mechanical microtomes costing in the thousands of dollars. The hand microtome is useful in simpler studies, and is very good as a teaching tool. Good hand microtomes sell for about thirty U.S. dollars. There are also some good plans out in the internet for making your own hand microtome in the home workshop. J.E. Paynter, 1907 "How to make a microtome" Amateur Work vol. 6 Draper Publishing Company is a really good instrument. I hope this is helpful. Yours sincerely Norman Woldow


Has cryogenic freezing been proven to work on any living thing?

Yes, on amphibians, reptiles and the like. No humans yet.