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An enzyme becomes denatured when:

A) the temperature exceeds the optimum temperature for that enzyme (ie the temperature that it works best at)

B) the pH of the surrounding of the enzyme is too low or too high for the optimum pH for that enzyme.

When enzymes are heated up too much they vibrate so vigorously that the bonds holding the protein structure in its specific shape becomes broken. The enzyme shape changes and the substrate no longer fits in to the active site. An enzyme which has become denatured is permanently inactive and will take no further part in reactions.

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June Douglas

Lvl 13
3y ago

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