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How does heating the bacterial smear promote entry of carbolfuchsin into the acid-fast cell wall?
Wiki User
∙ 14y ago
Since the acid-fast cell wall contains waxy mycolic acid, heat must be added in order for the carbol fuchsin stain to enter and remain in the cell wall (similar to if you were attempting to color a candle by melting the wax and adding a color dye to the liquefied wax).
Once the sample cools, the mycolic acid "hardens," so to say, and the stain remains locked into the cell wall through the acid alcohol wash and the methylene blue counterstain.
Wiki User
∙ 14y ago
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