Please I need the answer of this question
16S rRNA sequencing works by analyzing the genetic material of bacteria and other microorganisms to identify their species. This method targets a specific region of the 16S rRNA gene, which is unique to each species. By comparing the sequences obtained from a sample to a database of known sequences, scientists can determine the identity of the microbes present.
As it codes the small subunit of rRNA, so in evolutionary time course it has less mutations, less divergence, and mostly have retained the conserved old sequence. And this is the reason for which it is used in phylogenetic studies.
16S rRNA is used as a molecular marker to identify bacteria because it is a highly conserved gene that is present in all bacteria, allowing for comparisons between different species. This gene also contains regions that are unique to specific bacterial groups, making it a useful tool for distinguishing between different types of bacteria.
Biologists consider an organism's physical characteristics, genetics, and evolutionary history when classifying it into a specific group or category. These factors help determine an organism's relationships with other species and its place in the overall classification system.
Mitochondrial DNA codes for certain proteins that are essential for the function of the mitochondria, the cell's powerhouse. It also contains genes involved in energy production through oxidative phosphorylation. Mitochondrial DNA is separate from the nuclear DNA and is passed down maternally.
The 16s rRNA genes (rDNA) exist on genomic DNA. Therefore, plasmid has nothing to do with its amplification. However, if the 16s rRNA gene is cloned into the plasmid, it can be amplified.
16S rRNA sequencing works by analyzing the genetic material of bacteria and other microorganisms to identify their species. This method targets a specific region of the 16S rRNA gene, which is unique to each species. By comparing the sequences obtained from a sample to a database of known sequences, scientists can determine the identity of the microbes present.
As it codes the small subunit of rRNA, so in evolutionary time course it has less mutations, less divergence, and mostly have retained the conserved old sequence. And this is the reason for which it is used in phylogenetic studies.
Ribosomal 16S RNA found in the bacteria and small microorganisms prokaryotic cells and the subunit is 30S.
16S rRNA is used as a molecular marker to identify bacteria because it is a highly conserved gene that is present in all bacteria, allowing for comparisons between different species. This gene also contains regions that are unique to specific bacterial groups, making it a useful tool for distinguishing between different types of bacteria.
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16
Macrogen provides services such as standerd genetic sequencing. They also offer 16s rRNA full sequencing, microsatellite anlysis and difficult template sequencing.
4
16s + 4s = 20s
10 x 16 = 160
5.25 x 16 = 84