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if you got the question "how many molecules of DNA would result from one molecule after FIVE cycles of PCR?" then the answer is 32, not 16
Three.To see why, cut a piece of string in two places! Of course, strictly you would not be able to see only three fragments. You would amplify the DNA before carrying out electrophoresis. That way, you would get perhaps 200 million copies of each fragment, and they would show up. Also, you would only be able to distinguish the fragments if they were different lengths. Electrophoresis separates pieces of DNA by length.
After three cycles of PCR, the DNA would be amplified 8-fold because each cycle doubles the amount of DNA. So, starting with one molecule, after three cycles you would have 8 molecules of DNA.
A mutation that can be inherited by offspring would result from a change in the DNA sequence of a germ cell (sperm or egg) in the parent. This mutation would be present in the offspring's DNA and can be passed down to future generations.
DNA molecules treated with DNAase would undergo degradation, resulting in the cleavage of the phosphodiester bonds between nucleotides. This enzymatic activity would lead to the fragmentation of the DNA molecules into smaller pieces or nucleotides.
16
On a gel electrophoresis setup, small DNA pieces are expected to migrate further down the gel compared to larger DNA fragments. This is because smaller fragments can move more easily through the gel matrix, while larger fragments encounter more resistance. As a result, the smallest DNA pieces will be located closer to the bottom of the gel, while larger fragments will remain nearer to the wells where the samples were loaded.
Four.
if you got the question "how many molecules of DNA would result from one molecule after FIVE cycles of PCR?" then the answer is 32, not 16
Four.
Four.
DNA breaks into pieces and recombines during conception. Scientists also break DNA into pieces when studying it.
DNA-polymerase synthesizes DNA molecules, while DNA-ligase ca join DNA pieces together by making phosphodiester bond.
Four.
Four.
Four.
During electrophoresis, smaller pieces of DNA will migrate to the bottom of the gel first.