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1. An increasingly acidic environment (acidosis) causes a breakdown in the chemical reactions that produce muscle fibre contractions (acidosis is caused by the build-up of lactic acid and the release of hydrogen ions.) This is most likely to occur during high to intense levels of exercise.

2. Glucose reserves are depleted during sustained periods of work.

3. There is a change in the balance of the chemicals that propagate the nervous stimulus (the action potential) within the muscle fibre. This decreases the excitability of the muscle fibre and therefore reduces its ability to contract; it fatigues.

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