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1. REPLICATION: (nucleus)

- DNA unzips, using the enzyme, helicase, which unwinds it and breaks the hydrogen bonds.

- CBP (complimentary base pairing) occurs when DNA nucleotides move into place with A-T and G-C.

- The nucleotides rejoins, using the enzyme, DNA polymerase.

- Each double strand of DNA has one "parent" strand and on "daughter" strand, called semi-conservative replication (one old/one new).

2. TRANSCRIPTION: (nucleus)

- DNA unzips, using the enzyme, helicase, which unwinds it and breaks the hydrogen bonds.

- CBP (complimentary base pairing) occurs when RNA nucleotides move into place with A-U, T-A, G-C, and C-G.

- The RNA nucleotides rejoins, using the enzyme, RNA polymerase.

- Genes contain sections of DNA called introns, which are interruptions and are removed by ribozymes, and exons, which get expressed or become the protein.

- Once the mRNA has let the nucleus, it gets associated with ribosomes.

3. TRANSLATION: (ribosome)

- 3 steps...

a) Initiation: mRNA binds to smaller ribosomal subunit, then the larger subunit joins.

b) Elongation: polypeptide lengthens 1 amino acid at a time; the ribosome is large enough for 2 + RNA molecules, 1 coming, 1 outgoing.

c) Termination: a stop codon does not have a tRNA molecule so this allows the mRNA to "fall off" the ribosome and the ribosome to break into 2 subunits.

- Several ribosomes, called a polysome, move along a strand of mRNA at once, so several polypeptides of the same type can be made at once using one mRNA molecule.

4. PROTEIN: (ribosome)

- peptide bonds

- α-helix or β sheet by H-bond

- 3D structure covalent bonds between R-groups

- several polypeptides

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