What are the differences between bis-tris gel and tris-glycine gel in terms of their composition and performance in protein electrophoresis?

Answer 1

Bis-Tris gel and Tris-Glycine gel differ in their composition and performance in protein electrophoresis. Bis-Tris gel contains bis-tris propane buffer, which helps maintain a stable pH, while Tris-Glycine gel uses a tris-glycine buffer system. Bis-Tris gel is known for providing sharper protein bands and better resolution, making it ideal for separating proteins of similar sizes. Tris-Glycine gel, on the other hand, is more commonly used for routine protein electrophoresis due to its simplicity and lower cost.