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What is the difference between inter-assay and intra-assay variability in laboratory testing methods?
Inter-assay variability refers to differences in results between different tests, while intra-assay variability refers to variations within the same test.
What are the differences between BCA and Bradford assays for protein quantification?
The main difference between the BCA and Bradford assays for protein quantification is the mechanism by which they measure protein concentration. The BCA assay relies on the reduction of Cu2 to Cu1 by proteins, while the Bradford assay uses the binding of Coomassie dye to proteins. Additionally, the BCA assay is more sensitive and has a wider linear range compared to the Bradford assay.
What is the intra-assay coefficient of variation for this particular experiment?
The intra-assay coefficient of variation for this experiment is a measure of the variability within the same assay or test, indicating how consistent the results are.
Fiducial Limits in Microbiological Assay?
fiducial limits are similar to confidence limits. They contain the parameter of interest. But i m also not sure of the difference between the 2 limits. And the interpretation is same wether it's microbio or chem or bio.
If the mitochondrion is fundmental to the mtt assay which organelle plays a key role in the neutral red viability assay and why?
The lysosome plays a key role in the neutral red viability assay. Neutral red is taken up by lysosomes and accumulates in these acidic organelles in viable cells, producing a red color. This assay measures lysosomal function as an indicator of cell viability and health.
What is the difference between inter-assay and intra-assay variability in laboratory testing methods?
Inter-assay variability refers to differences in results between different tests, while intra-assay variability refers to variations within the same test.
What is the optimal beta-mercaptoethanol molarity for maintaining protein stability in a biochemical assay?
The optimal beta-mercaptoethanol molarity for maintaining protein stability in a biochemical assay is typically around 1-5 mM.
What is the optimal beta mercaptoethanol molarity for protein denaturation in a biochemical assay?
The optimal beta mercaptoethanol molarity for protein denaturation in a biochemical assay varies depending on the specific protein being studied. It is typically in the range of 1-10 mM.
What has the author Sakina Zerizer written?
Sakina Zerizer has written: 'Comparative biochemical study of peroxidase assay systems'
Why blocking buffer is used in Elisa?
Blocking buffer is used in ELISA to prevent non-specific binding of proteins or antibodies to the surface of the wells. By coating the surface with a blocking buffer, it helps to reduce background noise and increase the specificity of the assay by ensuring that the detection antibodies bind specifically to the target antigen.
What is an assay pound?
An assay pound is a small standard weight used in assaying bullion, which varies between assayers, but is generally around half a gram.
Why phosphate buffer is used in determination of enzyme activity?
Phosphate buffer is commonly used in enzyme assays because it maintains a stable pH (around 7.0) which is optimal for most enzyme activities. This helps to ensure that the enzyme functions at its maximum capacity and that the assay results are reliable. Additionally, phosphate buffer is compatible with many enzymatic reactions and is cost-effective.
What is protein assay?
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
What are the differences between BCA and Bradford assays for protein quantification?
The main difference between the BCA and Bradford assays for protein quantification is the mechanism by which they measure protein concentration. The BCA assay relies on the reduction of Cu2 to Cu1 by proteins, while the Bradford assay uses the binding of Coomassie dye to proteins. Additionally, the BCA assay is more sensitive and has a wider linear range compared to the Bradford assay.
Role of blocking buffer in latex agglutination?
Blocking buffer in latex agglutination helps to prevent nonspecific binding of the latex beads to surfaces or molecules other than the target antigen. This ensures that the reaction is specific and increases the sensitivity and accuracy of the assay. The blocking buffer also helps to minimize background noise and improve the signal-to-noise ratio of the test.
Is assay by mass balance equivalent to assay by as is basis?
No, assay by mass balance and assay by as is basis are not equivalent. Assay by mass balance calculates the amount of a component based on material balance equations, while assay by as is basis measures the amount of a component without accounting for any changes or losses that may occur during processing.
Why you add distilled water in restriction digestion of lambda DNA along with enzyme and assay buffer?
We generally add milliQ (DNase free) water. For digestion water is added usually. It provides right Hydrogen bonds between enzyme amino acid residues and bases present in the restriction sites. Utpal Roy
