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What else can I help you with?
What makes DNA visible?
You can an electrophoresis gel and then stain the gel using a solution such as coomassie blue to make the bands visible. Alternatively, you can stain a cell containing DNA by using acridine orange. It is necessary to observe these under an electron light microscope.
What is used to make the DNA visible on the gel?
YES!! You can use a simple Agarose gel to separate to view the DNA on electrophoresis. Use 0.8 - 1% gel for 5-10kbp , 2% for 0.2 - 1kbp. If the fragments are really tiny, use an Acrylamide gel (vertical gel) to electrophorese and they will show right out. This is to offset the instability of high concentration gels.
Function of agarose in agarose gel electrophoresis?
Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.
A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?
Run gel electrophoresis :: Apex
DNA isolated from water samples collected in six different sites was prepared and analyzed by gel electrophoresis for the presence of carp. The resulting gel is shown. Lanes 1 through 6 contain the DN?
Without an image or a description of the gel results, it is not possible to determine the presence of carp DNA in the water samples. Gel electrophoresis can separate DNA fragments based on size, but visual inspection is required to interpret the presence or absence of specific DNA bands corresponding to carp DNA. Additional information or analysis would be needed to make a conclusion regarding the presence of carp DNA in the water samples.
What makes DNA visible?
You can an electrophoresis gel and then stain the gel using a solution such as coomassie blue to make the bands visible. Alternatively, you can stain a cell containing DNA by using acridine orange. It is necessary to observe these under an electron light microscope.
Flouresecnt dyes are used in DNA electrophoresis?
Yes, fluorescent dyes are commonly used in DNA electrophoresis for visualizing DNA bands when they are exposed to ultraviolet (UV) light. These dyes bind to the DNA molecules to make them visible and easy to analyze. Examples of fluorescent dyes used in DNA electrophoresis include ethidium bromide and SYBR Green.
What is used to make the DNA visible on the gel?
YES!! You can use a simple Agarose gel to separate to view the DNA on electrophoresis. Use 0.8 - 1% gel for 5-10kbp , 2% for 0.2 - 1kbp. If the fragments are really tiny, use an Acrylamide gel (vertical gel) to electrophorese and they will show right out. This is to offset the instability of high concentration gels.
How do you avoid smearing of DNA bands in gel electrophoresis?
To avoid smearing of DNA bands in gel electrophoresis, ensure that the gel is properly prepared and poured to have an even surface. Use appropriate voltage and running conditions suitable for the size of DNA fragments being separated. Handle the gel carefully to prevent any unnecessary movement or disruption during loading and running.
The bands of color that make up white light are called a?
The bands of color that make up white light when combined are known as the visible light spectrum. They can be seen by the human eye when put through a prism.
How do you make DNA fingerprint?
gel electrophoresis
What is the reason for not getting DNA bands after electrophoresis?
A few reasons you may not see bands on the gel after electrophoresis:DNA concentration too low. More sample has to be loadedDNA sample is contaminated with RNADNA bands are too small and have run out of the gelThe potential (voltage) applied across the gel is not strong enoughThe buffer system in which the gel is suspended is not doing its job correctly. The buffer might have to be made fresh.The electrophoresis apparatus is not in the ocrrect orientation (electrodes not connected to the right poles)Additionally, there could also be other reasons like: improper DNA extraction procedure. If you are running a gel after PCR and still do not see bands, look into whether the DNA is being amplified correctly. See if you are using the correct primers.There are several factors that influence the electrophoresis technique. A close examination of the results obtained will help you make decisions about your future experimental approach.
How can you make custom made silicone bands?
you can normally go to a shop where they sell them and ask if they can either make you one or give you directions on where to get them done
Can you make loom bracelets with regular bands?
well yes you can I make them with hair bands and other bands
What is the main difference between protein electrophoresis and nucleic acid electrophoresis?
There are many similarities and differences between protein and DNA electrophoresis.Similarities:PAGE protein and DNA electrophoresis both cause separation by size, creating bands that are viewed by the scientist or a machine. The smallest segments more the fastest due to less friction with the surface of their medium or equipment.The movement of charges through the medium is what causes the DNA or proteins to move. Electrons move from the negative to positive end of the gel or capillary tube.Differences:In PAGE protein electrophoresis, a polyacrylamide gel is used to prevent convection from altering the movement of the proteins. If the proteins are charged, and there is a worry that the charge will affect the mobility of the protein segments, 1% SDS can be added to get rid of the mass/charge issue. This way, only the mass of the segment determines how far it moves. In DNA capillary electrophoresis, the size of the capillary is so small that it does not have room for convection to occur (it is only 20-50 microns wide). Thus, there is no medium in the capillary but DNA itself.In protein electrophoresis, the proteins are often dyed so their movement can be viewed with the naked eye, or a machine. With DNA capillary electrophoresis, DNA strands are made through DNA replication with dNTPs that are fluorescently labeled for the different nucleotides. Each base is labeled a different color. A fine laser lights up the DNA strand in the capillary tube and reads what color fluoresces. This is how the nucleotide is identified.Protein PAGE electrophoresis is used to determine the purity of a protein sample. It can also be used to see how large the chains are that make up a multi-chain protein if a denaturing agent is added. DNA electrophoresis is used to get the order of nucleotides in a DNA sequence. It is done by chopping the DNA sequence into many smaller bits and sequencing them, then putting them back together by lining them up according to sequence overlaps. This is called the "shotgun" method. Protein electrophoresis can figure out the order of about 15-20 amino acids by a similar method, but DNA electrophoresis can get up to 1000 nucleotides (~300 amino acids). DNA electrophoresis is limited by the low probability that the DNA sequence would be cut into a segment greater than 1000 nucleotides.
Does Silly Bands make bands for the Phillies?
Yes!
Who advertises advertisements?
Advertising is done by business organisation to make themselves visible and noticeable in a particular niche, so that sales can be increased.
