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Why is there an incubation step after the addition of the substrate in ELISA reactions?
There needs to be a conversion of the substrate for the chemical reaction to provide an H+ ion for the color change needed for ELISA. The temperature essentially lowers the activation energy to allow for this reaction to proceed.
Which step of a gram stain can be omitted without affecting determination of the reaction?
you can omit the last step - the safranin counterstain
What the second step in the Polymerase chain reaction process?
The second step in the Polymerase chain reaction (PCR) process is annealing. During annealing, the temperature is lowered to allow the primers to bind to the DNA template strands. This facilitates the specific targeting of the region to be amplified.
What is a self sustaining reaction called?
A self-sustaining reaction is called a chain reaction. In this type of reaction, the products of one step of the reaction serve as reactants in subsequent steps, creating a continuous cycle of reactions that can propagate indefinitely under certain conditions.
What molecule is the reactant in the first reaction of glycolysis?
http://wiki.answers.com/Q/What_molecule_is_the_reactant_in_the_first_reaction_of_glycolysis"
What does the rate determining step graph reveal about the reaction mechanism?
The rate determining step graph shows the slowest step in a reaction, which determines the overall rate of the reaction. This step often indicates the mechanism of the reaction, as it is typically the step with the highest activation energy.
What do we call each step that makes up a reaction mechanism?
Each step in a reaction mechanism is referred to as an elementary step.
Is the transition state the slowest step in a reaction mechanism?
The transition state is not a step in a reaction mechanism; it is a high-energy state that exists at the peak of the reaction potential energy diagram. The slowest step in a reaction mechanism is often referred to as the rate-determining step, which has the highest activation energy and determines the overall rate of the reaction.
What is the significance of the rate determining step in a reaction coordinate diagram?
The rate determining step in a reaction coordinate diagram is important because it determines the overall speed of the reaction. It is the slowest step in the reaction and sets the pace for the entire process. By understanding and optimizing the rate determining step, scientists can control and improve the efficiency of chemical reactions.
How is the molecularity of the rate-controlling step related to the overall reaction order?
The molecularity of the rate-controlling step may not necessarily be the same as the overall reaction order. The rate-controlling step is determined by the slowest step in a reaction mechanism, while the overall reaction order is the sum of the individual reactant concentrations in the rate law equation. It is possible for the molecularity of the rate-controlling step to influence the overall reaction order, but they are not always directly correlated.
What is the role of the rate determining step in the energy diagram of a chemical reaction?
The rate-determining step in a chemical reaction is the slowest step that determines the overall rate of the reaction. It sets the pace for the entire process and influences the energy diagram by determining the activation energy required for the reaction to occur.
Which is the committed step of glycolysis?
The committed step of glycolysis is the reaction catalyzed by phophofructokine (PFK) converting fructose-6-phosphate into fructose-1,6- bisphosphate. The reaction is irreversible and secondly, it's the only reaction peculiar to the glycolysis.
What role does the reaction coordinate diagram play in identifying the rate determining step of a chemical reaction?
The reaction coordinate diagram helps identify the rate determining step of a chemical reaction by showing the energy changes as the reaction progresses. The highest energy point on the diagram corresponds to the rate determining step, where the activation energy is highest.
How to determine the rate determining step from a graph?
To determine the rate-determining step from a graph, look for the slowest step where the rate of reaction is the lowest. This step will have the highest activation energy and will be the one that controls the overall rate of the reaction.
How can one determine the rate-determining step from a graph?
To determine the rate-determining step from a graph, look for the slowest step where the rate of reaction is the lowest. This step will have the highest activation energy and will be the one that controls the overall rate of the reaction.
What is purpose of redox reaction in the cell?
To rlease energy slowly in a step-by-step process
X is second order and Y is first order X plus Y-- XY Does the reaction occur in a single step in which X and Y collide?
Yes, the reaction occurs in the step where X and Y collide. The first step is part of an elementary reaction and in an elementary reaction, the order is nothing but the coefficient in front of the reactant.
